DNA replication with the mutagenic nucleotide dPTP; Effects of tautomerism on nucleotide incorporation

被引：0
作者：
Harris, VH [1 ]
Smith, C [1 ]
Cummins, J [1 ]
Hamilton, A [1 ]
Hornby, DP [1 ]
Williams, DM [1 ]
机构：
[1] Univ Sheffield, Dept Chem, Krebs Inst, Ctr Chem Biol, Sheffield S3 7HF, S Yorkshire, England
来源：
CHEMISTRY OF NUCLEIC ACID COMPONENTS | 2002年 / 5卷
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The tautomeric constant of the nucleoside analogue P has been calculated and the kinetic parameters for the incorporation of dPTP by exo-Klenow have been determined. A strong correlation between the two suggests a role for the minor tautomeric forms of the bases in substitution mutagenesis during DNA replication.
引用
收藏
页码：215 / 220
页数：6
相关论文
共 50 条
  • [21] dRTP and dPTP a complementary nucleotide couple for the Sequence Saturation Mutagenesis (SeSaM) method
    Ruff, Anna Joelle
    Marienhagen, Jan
    Verma, Rajni
    Roccatano, Danilo
    Genieser, Hans-Gottfried
    Niemann, Percy
    Shivange, Amol V.
    Schwaneberg, Ulrich
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2012, 84 : 40 - 47
  • [22] Pol η is required for DNA replication during nucleotide deprivation by hydroxyurea
    S de Feraudy
    C L Limoli
    E Giedzinski
    D Karentz
    T M Marti
    L Feeney
    J E Cleaver
    Oncogene, 2007, 26 : 5713 - 5721
  • [23] NAD+ regulates nucleotide metabolism and genomic DNA replication
    Sebastian Howen Nesgaard Munk
    Joanna Maria Merchut-Maya
    Alba Adelantado Rubio
    Arnaldur Hall
    George Pappas
    Giacomo Milletti
    MyungHee Lee
    Lea Giørtz Johnsen
    Per Guldberg
    Jiri Bartek
    Apolinar Maya-Mendoza
    Nature Cell Biology, 2023, 25 : 1774 - 1786
  • [24] Effects of a modified dye-labeled nucleotide spacer arm on incorporation by thermophilic DNA polymerases
    Lacenere, CJ
    Garg, NK
    Stoltz, BM
    Quake, SR
    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2006, 25 (01) : 9 - 15
  • [25] The effect of tautomeric constant on the specificity of nucleotide incorporation during DNA replication: Support for the rare tautomer hypothesis of substitution mutagenesis
    Harris, VH
    Smith, CL
    Cummins, WJ
    Hamilton, AL
    Adams, H
    Dickman, M
    Hornby, DP
    Williams, DM
    JOURNAL OF MOLECULAR BIOLOGY, 2003, 326 (05) : 1389 - 1401
  • [26] Nucleotide-induced DNA polymerase active site motions accommodating a mutagenic DNA intermediate
    Batra, VK
    Beard, WA
    Shock, DD
    Pedersen, LC
    Wilson, SH
    STRUCTURE, 2005, 13 (08) : 1225 - 1233
  • [27] INCORPORATION OF A FLUORESCENT NUCLEOTIDE INTO OLIGORIBONUCLEOTIDES
    ADAMS, CJ
    MURRAY, JB
    ARNOLD, JRP
    STOCKLEY, PG
    TETRAHEDRON LETTERS, 1994, 35 (10) : 1597 - 1600
  • [28] Effects of exonuclease activity and nucleotide selectivity of the herpes simplex virus DNA polymerase on the fidelity of DNA replication in vivo
    Hwang, YT
    Liu, BY
    Hong, CY
    Shillitoe, EJ
    Hwang, CBC
    JOURNAL OF VIROLOGY, 1999, 73 (07) : 5326 - 5332
  • [29] NUCLEOTIDE INCORPORATION OPPOSITE DEGENERATE BASES BY TAQ DNA-POLYMERASE
    KAMIYA, H
    MURATAKAMIYA, N
    LIN, PKT
    BROWN, DM
    OHTSUKA, E
    NUCLEOSIDES & NUCLEOTIDES, 1994, 13 (6-7): : 1483 - 1492
  • [30] Nucleotide pools dictate the identity and frequency of ribonucleotide incorporation in mitochondrial DNA
    Berglund, Anna-Karin
    Navarrete, Clara
    Engqvist, Martin K. M.
    Hoberg, Emily
    Szilagyi, Zsolt
    Taylor, Robert W.
    Gustafsson, Claes M.
    Falkenberg, Maria
    Clausen, Anders R.
    PLOS GENETICS, 2017, 13 (02):
12345