Expression of a family of noncoding mitochondrial RNAs distinguishes normal from cancer cells

被引：103

作者：

Burzio, Veronica A. ^{[1
,2
,3
,4
]}

Villota, Claudio ^{[1
,2
,3
,4
]}

Villegas, Jaime ^{[1
,2
,3
,4
]}

Landerer, Eduardo ^{[1
,3
,4
]}

Boccardo, Enrique ^{[5
]}

Villa, Luisa L. ^{[5
]}

Martinez, Ronny ^{[3
]}

Lopez, Constanza ^{[1
,3
]}

Gaete, Fancy ^{[6
]}

Toro, Viviana ^{[7
]}

Rodriguez, Ximena ^{[7
]}

Burzio, Luis O. ^{[1
,2
,3
,4
]}

机构：

[1] Andes Biosci SA, Santiago 7780272, Chile

[2] Grp Bios SA, Santiago 7780272, Chile

[3] Fdn Ciencia Vida, Santiago 7780272, Chile

[4] Univ Andres Bello, Dept Biol Sci, Santiago 8370134, Chile

[5] Ludwig Inst Canc Res, Virol Lab, BR-01323903 Sao Paulo, Brazil

[6] Hosp Luis Tisne Brousse, Pathol Serv, Santiago 7930124, Chile

[7] Hosp San Jose, Pathol Serv, Santiago 8380419, Chile

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 23期

关键词：

differential expression in cancer; RNAs with stem-loop structures; IN-SITU HYBRIDIZATION; DNA-REPLICATION; FIXATION; NUCLEUS;

D O I：

10.1073/pnas.0903086106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We reported the presence in human cells of a noncoding mitochondrial RNA that contains an inverted repeat (IR) of 815 nucleotides (nt) covalently linked to the 5' end of the mitochondrial 16S RNA (16S mtrRNA). The transcript contains a stem-loop structure and is expressed in human proliferating cells but not in resting cells. Here, we demonstrate that, in addition to this transcript, normal human proliferating cells in culture express 2 antisense mitochondrial transcripts. These transcripts also contain stem-loop structures but strikingly they are down-regulated in tumor cell lines and tumor cells present in 17 different tumor types. The differential expression of these transcripts distinguishes normal from tumor cells and might contribute a unique vision on cancer biology and diagnostics.

引用

页码：9430 / 9434

页数：5

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