Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy

被引：7

作者：

Ma, Xiande ^{[1
,2
]}

Zhang, Wei ^{[3
]}

Xu, Chang ^{[4
]}

Zhang, Shuangshuang ^{[4
]}

Zhao, Jiaxiu ^{[4
]}

Pan, Qian ^{[5
]}

Wang, Zhe ^{[5
]}

机构：

[1] Liaoning Univ Tradit Chinese Med, Teaching & Expt Ctr, Shenyang 110847, Peoples R China

[2] Liaoning Univ Tradit Chinese Med, Key Lab, Minist Educ TCM Viscera State Theory & Applicat, Shenyang 110847, Peoples R China

[3] Liaoning Univ Tradit Chinese Med, Dept Encephalopathy Rehabil 3, Affiliated Hosp 1, Shenyang 110032, Peoples R China

[4] Liaoning Univ Tradit Chinese Med, Dept Basic Sci Integrated Chinese & Western Med, Shenyang 110847, Peoples R China

[5] Liaoning Univ Tradit Chinese Med, Coll Integrated Chinese & Western Med, Dept Pathol, Shenyang 110847, Peoples R China

来源：

EXPERIMENTAL AND MOLECULAR PATHOLOGY | 2020年 / 117卷

基金：

中国国家自然科学基金;

关键词：

Cerebral ischemia/reperfusion injury; Nucleotide-binding oligomerization domain 1; Autophagy; Endoplasmic reticulum stress; Oxygen-glucose deprivation and reperfusion; MYOCARDIAL ISCHEMIA/REPERFUSION INJURY; ER STRESS; CEREBRAL-ISCHEMIA; NOD1; INFLAMMATION;

D O I：

10.1016/j.yexmp.2020.104525

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Cerebral ischemia-reperfusion (CIR) can regulate multiple transcription factors to enhance or attenuate injury. Nucleotide-binding oligomerization domain protein 1 (NOD1) has been reported to be involved in autophagy and endoplasmic reticulum (ER) stress. Moreover, autophagy and ER stress play important roles in CIR injury. Hence, the function of NOD1 in CIR injury was explored in this study. Primary rat cortical neurons were treated with oxygen-glucose deprivation and reperfusion (OGD/R) in vitro. NOD1 level was measured using immunofluorescence, real-time quantitative PCR and western blotting and its ubiquitination using co-immunoprecipitation. Results showed that OGD/R up-regulated NOD1 level but inhibited NOD1 ubiquitination. Then the effect of NOD1 on OGD/R-induced changes in cell viability, apoptosis, autophagy and ER stress was evaluated by methyl thiazolyl tetrazolium assay, lactate dehydrogenase release, Hoechst staining, detection of autophagy and ER stress-related proteins using western blotting and infection with GFP-LC3 lentiviruses. OGD/R decreased cell viability and increased cell apoptosis. NOD1 up-regulation promoted these changes, but NOD1 down-regulation reversed these changes. Moreover, OGD/R triggered autophagy and ER stress and NOD1 silencing reversed OGD/R-induced changes in autophagy and ER stress. To validate the role of autophagy in OGD/ R injury, autophagy inducer rapamycin was used. Rapamycin promoted OGD/R-induced decrease in cell viability and counteracted NOD1 silencing-induced increase in cell viability. In addition, ER stress inducer tunicamycin was used to investigate the relationship between ER stress and autophagy. Tunicamycin promoted OGD/R-induced decrease in cell viability and reversed NOD1 silencing-induced increase in cell viability. Tunicamycin also enhanced OGD/R-induced autophagy and reversed NOD1 silencing-induced inhibition in autophagy. The results indicated that NOD1 promoted OGD/R injury in cortical neurons through activating ER stress-mediated autophagy. This study provides new insights for the target of CIR injury treatment.

引用

页数：8

共 33 条

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