Microbial diversity of anammox bacteria enriched from different types of seed sludge in an anaerobic continuous-feeding cultivation reactor

被引：38

作者：

Date, Yasuhiro ^{[1
]}

Isaka, Kazuichi ^{[2
]}

Ikuta, Hajime ^{[2
]}

Sumino, Tatsuo ^{[2
]}

Kaneko, Naoya ^{[1
]}

Yoshie, Sachiko ^{[1
]}

Tsuneda, Satoshi ^{[1
]}

Inamori, Yuhei ^{[3
]}

机构：

[1] Waseda Univ, Dept Life Sci & Med BioSci, Shinjuku Ku, Tokyo 1628480, Japan

[2] Hitachi Plant Technol, Chiba 2710064, Japan

[3] Natl Inst Environm Studies, Tsukuba, Ibaraki 3050053, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2009年 / 107卷 / 03期

关键词：

Anaerobic ammonium oxidation (anammox); Microbial diversity; Nonwoven fabric carrier; Nitrogen removal; Phylogenetic analysis; Reproducible enrichment; AMMONIUM-OXIDIZING BACTERIA; FLUIDIZED-BED REACTOR; REMOVAL PERFORMANCE; NITROGEN REMOVAL; WASTE-WATER; OXIDATION; CARRIER;

D O I：

10.1016/j.jbiosc.2008.11.015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enrichment of anammox bacteria from three types of seed sludge, sewage, digester, and nitrification sludges, was conducted using a nonwoven fabric carrier for immobilizing the anammox bacteria, and the microbial diversity of the enriched anammox culture was investigated. About four months later, simultaneous removals of ammonium and nitrite, and production of a small amount of nitrate, which is unique to the anammox reaction, were observed in all 3 sludge reactors. Results of 16S rRNA gene analysis indicated that anammox bacteria were cultivated and diversified in each sludge type. Moreover, the microbial diversity of anammox bacteria was higher in the enriched culture from sewage sludge compared to the other two types of seed sludge. Bacillus sp. coexisted in the anammox culture cultivated from sewage sludge. These results suggest that differences in the anammox community in the enriched culture were caused by differences in the type of seed sludge. (C) 2008, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：281 / 286

页数：6

共 22 条

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