The Role of Microclot Formation in an Acute Subarachnoid Hemorrhage Model in the Rabbit

被引:19
作者
Andereggen, Lukas [1 ,2 ,3 ]
Neuschmelting, Volker [4 ,5 ]
von Gunten, Michael [6 ]
Widmer, Hans Rudolf [1 ]
Fandino, Javier [4 ,7 ]
Marbacher, Serge [4 ,7 ]
机构
[1] Univ Hosp Bern, Inselspital Bern, Dept Neurosurg, CH-3012 Bern, Switzerland
[2] Boston Childrens Hosp, Labs Neurosci Res Neurosurg, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Boston, MA 02115 USA
[4] Univ Hosp Bern, Inselspital Bern, Dept Intens Care Med, CH-3012 Bern, Switzerland
[5] Univ Hosp Cologne, Dept Neurosurg, D-50924 Cologne, Germany
[6] Inst Pathol Langgasse, CH-3012 Bern, Switzerland
[7] Kantonsspital Aarau, Dept Neurosurg, CH-5001 Aarau, Switzerland
关键词
EARLY BRAIN-INJURY; GLOBAL CEREBRAL ATROPHY; INTRACRANIAL-PRESSURE; ANIMAL-MODELS; BLOOD-FLOW; MICROTHROMBOSIS; MECHANISMS; CONSTRICTION; INFLAMMATION; COAGULATION;
D O I
10.1155/2014/161702
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background. Microvascular dysfunction and microthrombi formation are believed to contribute to development of early brain injury (EBI) after aneurysmal subarachnoid hemorrhage (SAH). Objective. This study aimed to determine (i) extent of microthrombus formation and neuronal apoptosis in the brain parenchyma using a blood shunt SAH model in rabbits; (ii) correlation of structural changes in microvessels with EBI characteristics. Methods. Acute SAH was induced using a rabbit shunt cisterna magna model. Extent of microthrombosis was detected 24 h post-SAH (n = 8) by fibrinogen immunostaining, compared to controls (n = 4). We assessed apoptosis by terminal deoxynucleotidyl transferase nick end labeling (TUNEL) in cortex and hippocampus. Results. Our results showed significantly more TUNEL-positive cells (SAH: 115 +/- 13; controls: 58 +/- 10; P = 0.016) and fibrinogen-positive microthromboemboli (SAH: 9 +/- 2; controls: 2 +/- 1;P = 0.03) in the hippocampus after aneurysmal SAH. Conclusions. We found clear evidence of early microclot formation in a rabbit model of acute SAH. The extent of microthrombosis did not correlate with early apoptosis or CPP depletion after SAH; however, the total number of TUNEL positive cells in the cortex and the hippocampus significantly correlated with mean CPP reduction during the phase of maximum depletion after SAH induction. Both microthrombosis and neuronal apoptosis may contribute to EBI and subsequent DCI.
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页数:10
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