An NBD-NH2 fluorescent probe for bioimaging: existence of a specific detection of ClO-

被引：7

作者：

Yang, Yunxia ^{[1
]}

Zhang, Di ^{[2
]}

Xu, Mengsheng ^{[1
]}

Wang, Jianhong ^{[1
]}

Chen, Jipeng ^{[1
]}

Wang, Le ^{[1
]}

机构：

[1] Shanghai Univ Engn Sci, Coll Chem & Chem Engn, Shanghai 201620, Peoples R China

[2] Henan Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Henan Cereal Qual & Safety Testing Key Lab, Zhengzhou 450002, Henan, Peoples R China

来源：

MONATSHEFTE FUR CHEMIE | 2018年 / 149卷 / 06期

基金：

上海市自然科学基金;

关键词：

NBD-NH2; Fluorescent probe; Hypochlorous; HYPOCHLOROUS ACID; OXIDATIVE STRESS; HYDROGEN-SULFIDE; LIVING CELLS; RHODAMINE-B; LIVE CELLS; IN-VIVO; CHEMOSENSOR; RECOGNITION; SENSOR;

D O I：

10.1007/s00706-018-2143-9

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A fluorescent probe 4-amino-7-nitrobenz-2-oxa-1,3-diazole NBD-NH2 for critical sensing hypochlorous (ClO-) has been developed in this paper. Probe NBD-NH2 exhibited excellent sensitivity and high selectivity toward detection of ClO- in CH3CH2OH/HEPES solution (1:4, v/v, 10 mM, pH 7.4), where the detection limit as low as 40 nM. The green fluorescence of NBD-NH2 was distinctly quenched by addition of ClO-. In addition, the new probe was also applied for bioimaging using HeLa cells and exist a critical detection of ClO- in living cells. [GRAPHICS] .

引用

页码：1003 / 1008

页数：6

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