The major antennal chemosensory protein of red imported fire ant workers

被引：68

作者：

Gonzalez, D. ^{[1
]}

Zhao, Q. ^{[1
]}

McMahan, C. ^{[1
]}

Velasquez, D. ^{[1
]}

Haskins, W. E. ^{[1
,2
]}

Sponsel, V. ^{[1
]}

Cassill, A. ^{[1
]}

Renthal, R. ^{[1
,2
]}

机构：

[1] Univ Texas San Antonio, Dept Biol, San Antonio, TX 78249 USA

[2] Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem, San Antonio, TX 78229 USA

来源：

INSECT MOLECULAR BIOLOGY | 2009年 / 18卷 / 03期

基金：

美国国家卫生研究院;

关键词：

chemosensory proteins; Solenopsis invicta; proteomics; cuticular lipids; cyclodextrin; PHEROMONE-BINDING PROTEIN; SOLENOPSIS-INVICTA BUREN; CUTICULAR HYDROCARBONS; SOLUBLE-PROTEINS; SWISS-MODEL; GENE FAMILY; HONEY-BEE; INSECT; EXPRESSION; RECOGNITION;

D O I：

10.1111/j.1365-2583.2009.00883.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Some chemosensory proteins (CSPs) are expressed in insect sensory appendages and are thought to be involved in chemical signalling by ants. We identified 14 unique CSP sequences in expressed sequence tag (EST) libraries of the red imported fire ant, Solenopsis invicta. One member of this group (Si-CSP1) is highly expressed in worker antennae, suggesting an olfactory function. A shotgun proteomic analysis of antennal proteins confirmed the high level of Si-CSP1 expression, and also showed expression of another CSP and two odorant-binding proteins (OBPs). We cloned and expressed the coding sequence for Si-CSP1. We used cyclodextrins as solubilizers to investigate ligand binding. Fire ant cuticular lipids strongly inhibited Si-CSP1 binding to the fluorescent dye N-phenyl-naphthylamine, suggesting cuticular substances are ligands for Si-CSP1. Analysis of the cuticular lipids showed that the endogenous ligands of Si-CSP1 are not cuticular hydrocarbons.

引用

页码：395 / 404

页数：10

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