Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coli and their fimbriae

被引：1

作者：

Goetstouwers, Tiphanie ^{[1
]}

Van Poucke, Mario ^{[1
]}

Coddens, Annelies ^{[2
]}

Van Ut Nguyen ^{[2
]}

Melkebeek, Vesna ^{[2
]}

Deforce, Dieter ^{[3
]}

Cox, Eric ^{[2
]}

Peelman, Luc J. ^{[1
]}

机构：

[1] Univ Ghent, Fac Vet Med, Lab Anim Genet, B-9820 Merelbeke, Belgium

[2] Univ Ghent, Fac Vet Med, Lab Immunol, B-9820 Merelbeke, Belgium

[3] Univ Ghent, Fac Pharmaceut Sci, Lab Pharmaceut Biotechnol, B-9000 Ghent, Belgium

来源：

BMC GENETICS | 2014年 / 15卷

关键词：

F4 Escherichia coli; Glycosphingolipids; Pig; Variation; Binding; PIGS; PHENOTYPES; K88; SUSCEPTIBILITY; EXPRESSION; ADHESIVE; DIARRHEA; PIGLETS; F4AB/AC; K99;

D O I：

10.1186/s12863-014-0103-x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Glycosphingolipids (GSLs) are important membrane components composed of a carbohydrate structure attached to a hydrophobic ceramide. They can serve as specific membrane receptors for microbes and microbial products, such as F4 Escherichia coli (F4 ETEC) and isolated F4 fimbriae. The aim of this study was to investigate the hypothesis that variation in genes involved in the assembly of the F4 binding carbohydrate moiety of GSLs (i.e. ARSA, B4GALT6, GAL3ST1, GALC, GBA, GLA, GLB1, GLB1L, NEU1, NEU2, UGCG, UGT8) could account for differential binding of F4 ETEC and their fimbriae. Results: RT-PCR could not reveal any differential expression of the 12 genes in the jejunum of F4 receptor-positive (F4R(+)) and F4 receptor-negative (F4R(-)) pigs. Sequencing the complete open reading frame of the 11 expressed genes (NEU2 was not expressed) identified 72 mutations. Although some of them might have a structural effect, none of them could be associated with a F4R phenotype. Conclusion: We conclude that no regulatory or structural variation in any of the investigated genes is responsible for the genetic susceptibility of pigs towards F4 ETEC.

引用

页数：5

共 32 条

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