Molecular analysis of system N suggests novel physiological roles in nitrogen metabolism and synaptic transmission

被引：274

作者：

Chaudhry, FA

Reimer, RJ

Krizaj, D

Barber, D

Storm-Mathisen, J

Copenhagen, DR

Edwards, RH ^{[1
]}

机构：

[1] Univ Calif San Francisco, Sch Med, Grad Program Neurosci, Dept Neurol, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Sch Med, Grad Program Cell Biol, Dept Neurol, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Sch Med, Grad Program Biomed Sci, Dept Neurol, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Sch Med, Dept Ophthalmol, San Francisco, CA 94143 USA

[5] Univ Calif San Francisco, Sch Med, Dept Stomatol, San Francisco, CA 94143 USA

[6] Univ Calif San Francisco, Sch Med, Dept Surg, San Francisco, CA 94143 USA

[7] Univ Calif San Francisco, Sch Med, Dept Physiol, San Francisco, CA 94143 USA

[8] Univ Oslo, Inst Basic Med Sci, Dept Anat, N-0317 Oslo, Norway

来源：

CELL | 1999年 / 99卷 / 07期

关键词：

D O I：

10.1016/S0092-8674(00)81674-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amino acid glutamine has a central role in nitrogen metabolism. Although the molecular mechanisms responsible for its transport across cell membranes remain poorly understood, classical amino acid transport system N appears particularly important. Using intracellular pH measurements, we have now identified an orphan protein related to a vesicular neurotransmitter transporter as system N. Functional analysis shows that this protein (SN1) involves H+ exchange as well as Na+ cotransport and, under physiological conditions, mediates glutamine efflux as well as uptake. Together with the pattern of SN1 expression, these unusual properties suggest novel physiological roles for system N in nitrogen metabolism and synaptic transmission.

引用

页码：769 / 780

页数：12

共 52 条

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