TcZFP1:: a CCCH zinc finger protein of Trypanosoma cruzi that binds poly-C oligoribonucleotides in vitro

被引：17
作者：
Mörking, PA
Dallagiovanna, BM
Foti, L
Garat, B
Picchi, GFA
Umaki, ACS
Probst, CM
Krieger, MA
Goldenberg, S
Fragoso, SP
机构：
[1] Inst Biol Mol Parana, BR-81350010 Curitiba, Parana, Brazil
[2] Fiocruz MS, IOC, BR-21045900 Rio De Janeiro, Brazil
[3] Univ Republica, Fac Ciencias, Lab Interacciones Mol, Montevideo 11400, Uruguay
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 319卷 / 01期
关键词：
Trypanosoma cruzi; CCCH zinc finger protein; metacyclogenesis; EMSA;
D O I：
10.1016/S0006-291X(04)00873-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have identified two zinc finger proteins of Trypanosoma cruzi, the protozoan parasite that causes Chagas disease in humans. These proteins, named tcZFP1 and tcZFP2, share the unusual zinc finger motif (CCCH) found in a diverse range of RNA-binding proteins involved in various aspects of the control of cell homeostasis and differentiation. We report here the functional expression of a recombinant tcZFP1, and the relative affinity and stability of the specific complexes formed between the protein and synthetic oligoribonucleotides containing C-rich sequences. (C) 2004 Elsevier Inc. All rights reserved.
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收藏
页码：169 / 177
页数：9
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