Label-free silver nanoparticles for the naked eye detection of entecavir

被引：25

作者：

Gao, Mengmeng ^{[1
]}

Lin, Rui ^{[2
]}

Li, Lili ^{[5
]}

Jiang, Li ^{[3
]}

Ye, Baofen ^{[1
]}

He, Hua ^{[1
,4
]}

Qiu, Lanlan ^{[2
]}

机构：

[1] China Pharmaceut Univ, Div Analyt Chem, Nanjing 210009, Jiangsu, Peoples R China

[2] Yancheng Hlth Vocat & Tech Coll, Yancheng 224005, Peoples R China

[3] Jiangxi Canc Hosp, Dept Pharm, Nanchang 330029, Peoples R China

[4] China Pharmaceut Univ, Key Lab Drug Qual Control & Pharmacovigilance, Minist Educ, Nanjing 210009, Jiangsu, Peoples R China

[5] Pingdingshan Univ, Sch Med, Pingdingshan 467000, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2014年 / 126卷

关键词：

Entecavir; Naked eye colorimetric detection; Human urine; Label-free; Silver nanoparticles; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; BETA-CYCLODEXTRIN; HYDROGEN-PEROXIDE; VISUAL DETECTION; HUMAN PLASMA; THERAPY; PH; COMBINATION; MELAMINE;

D O I：

10.1016/j.saa.2014.02.009

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A simple, rapid, field-portable colorimetric method for the detection of entecavir was proposed based on the color change caused by the aggregation of silver nanoparticles. Neutralization of the electrostatic repulsion from each silver nanoparticle resulted in the aggregation of AgNPs and a consequent color change of AgNPs from yellow to wine-red, which provided a platform for rapid and field-portable colorimetric detection of entecavir. The concentration of entecavir could be determined with naked eye or UV-vis spectrometer. The proposed method can be used to detect entecavir in human urine with a detection limit of 1.51 jig mL(-1), within 25 min by naked eye observation without the aid of any advanced instrument or complex pretreatment. Results from UV-vis spectra showed that the absorption ratio was linear with the concentration of entecavir in the range of 5.04-25.2 mu g mL(-1) and 1.015.04 mu g mL(-1) with linear coefficients of 0.9907 and 0.9955, respectively. The selectivity of AgNPs detection system for entecavir is excellent comparing with other ions and analytes. Due to its rapid, visible color changes, and excellent selectivity, the AgNPs synthesized in this study are suitable to be applied to on-site screening of entecavir in human urine. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：178 / 183

页数：6

共 29 条

[1] LC-ESI-MS/MS method for the quantification of entecavir in human plasma and its application to bioequivalence study [J].

Challa, Balasekhara Reddy ;

Awen, Bahlul Z. ;

Chandu, Babu Rao ;

Rihanaparveen, Shaik .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (11-12) :769-776

[2] Applications of nanomaterials in enantioseparation and related techniques [J].

Chang, Cuilan ;

Wang, Xin ;

Bai, Yu ;

Liu, Huwei .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2012, 39 :195-206

[3] Detection of Trace Nitroaromatic Isomers Using Indium Tin Oxide Electrodes Modified Using β-Cyclodextrin and Silver Nanoparticles [J].

Chen, Xin ;

Cheng, Xiaoyu ;

Gooding, J. Justin .

ANALYTICAL CHEMISTRY, 2012, 84 (20) :8557-8563

[4] β-Cyclodextrin-Functionalized Silver Nanoparticles for the Naked Eye Detection of Aromatic Isomers [J].

Chen, Xin ;

Parker, Stephen G. ;

Zou, Gang ;

Su, Wei ;

Zhang, Qijin .

ACS NANO, 2010, 4 (11) :6387-6394

[5] Impact of Environmental Conditions (pH, Ionic Strength, and Electrolyte Type) on the Surface Charge and Aggregation of Silver Nanoparticles Suspensions [J].

El Badawy, Amro M. ;

Luxton, Todd P. ;

Silva, Rendahandi G. ;

Scheckel, Kirk G. ;

Suidan, Makram T. ;

Tolaymat, Thabet M. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2010, 44 (04) :1260-1266

[6] A new rapid colorimetric detection method of Mn2+ based on tripolyphosphate modified silver nanoparticles [J].

Gao, Yue-Xia ;

Xin, Jun-Wei ;

Shen, Zhe-Yu ;

Pan, Wei ;

Li, Xing ;

Wu, Ai-Guo .

SENSORS AND ACTUATORS B-CHEMICAL, 2013, 181 :288-293

[7] Visual detection of melamine in milk products by label-free gold nanoparticles [J].

Guo, Liangqia ;

Zhong, Jianhai ;

Wu, Jinmei ;

Fu, FengFu ;

Chen, Guonan ;

Zheng, Xiaoyan ;

Lin, Song .

TALANTA, 2010, 82 (05) :1654-1658

[8] Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage [J].

Hudecova, Alexandra ;

Kusznierewicz, Barbara ;

Hasplova, Katarina ;

Huk, Anna ;

Magdolenova, Zuzana ;

Miadokova, Eva ;

Galova, Eliska ;

Dusinska, Maria .

FOOD AND CHEMICAL TOXICOLOGY, 2012, 50 (09) :3352-3359

[9] Efficacy of Entecavir and Adefovir Combination Therapy for Patients with Lamivudine- and Entecavir-Resistant Chronic Hepatitis B [J].

Jeon, Jung Won ;

Shin, Hyun Phil ;

Lee, Joung Il ;

Joo, Kwang Ro ;

Cha, Jae Myung ;

Park, Jae Jun ;

Lim, Jun Uk ;

Lim, Kyuseong ;

Kim, Sunyong .

DIGESTIVE DISEASES AND SCIENCES, 2012, 57 (05) :1358-1365

[10] Oxidative DNA damage corresponds to the long term survival of human cells treated with silver nanoparticles [J].

Kruszewski, Marcin ;

Gradzka, Iwona ;

Bartlomiejczyk, Teresa ;

Chwastowska, Jadwiga ;

Sommer, Sylwester ;

Grzelak, Agnieszka ;

Zuberek, Mariusz ;

Lankoff, Anna ;

Dusinska, Maria ;

Wojewodzka, Maria .

TOXICOLOGY LETTERS, 2013, 219 (02) :151-159

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