Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

被引：14

作者：

Ali, Aysha ^{[1
]}

Bullen, Gemma A. ^{[1
]}

Cross, Benjamin ^{[1
]}

Dafforn, Timothy R. ^{[2
]}

Little, Haydn A. ^{[1
]}

Manchester, Jack ^{[1
]}

Peacock, Anna F. A. ^{[1
]}

Tucker, James H. R. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England

[2] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 39期

基金：

英国工程与自然科学研究理事会;

关键词：

PHOTOCONTROL; BINDING; PHOTODIMERIZATION; INHIBITION; PEPTIDES; MOLECULE;

D O I：

10.1039/c9cc01540j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The reversible photocontrol of an enzyme governing blood coagulation is demonstrated. The thrombin binding aptamer (TBA), was rendered photochromic by modification with two anthracene groups. Lighttriggered anthracene photodimerisation distorts its structure, inhibiting binding of the enzyme thrombin, which in turn triggers catalysis and the resulting clotting process.

引用

页码：5627 / 5630

页数：4

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