Isolation of adipose-derived stem cells: a comparison among different methods

被引：94

作者：

Markarian, Carolina Franke ^{[1
]}

Frey, Gianna Zaffari ^{[2
]}

Silveira, Maiele Dornelles ^{[2
]}

Chem, Eduardo Mainieri ^{[1
]}

Milani, Adriana Rosa ^{[1
]}

Ely, Pedro Bins ^{[1
]}

Horn, Ana Paula ^{[3
]}

Nardi, Nance Beyer ^{[2
]}

Camassola, Melissa ^{[2
]}

机构：

[1] Complexo Hosp Santa Casa Porto Alegre, BR-90020090 Porto Alegre, RS, Brazil

[2] Univ Luterana Brasil, Lab Stem Cells & Tissue Engn, BR-92425900 Canoas, RS, Brazil

[3] Univ Fed Rio Grande, Histol Lab, BR-96203900 Rio Grande, RS, Brazil

来源：

BIOTECHNOLOGY LETTERS | 2014年 / 36卷 / 04期

关键词：

Adipose-derived stromal cell; Bone tissue engineering; Isolation methods; Mesenchymal stem cells; Osteogenic differentiation; Trypsin digestion; MESENCHYMAL STROMAL CELLS; OSTEOGENIC DIFFERENTIATION; INTERNATIONAL-SOCIETY; BONE-MARROW; TISSUE; BLOOD;

D O I：

10.1007/s10529-013-1425-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Adipose-derived stromal cells (ASCs) are usually isolated by digestion with collagenase. We have compared alternative methods to isolate ASCs in a more economically viable protocol. Nine protocols using red blood cells lysis buffer solution, trypsin, collagenase and centrifugation were compared; the isolation rate, cell viability, expansion rate, immunophenotype and differentiation in adipogenic and osteogenic lineages were analyzed. ASCs were isolated and successfully maintained by digestion with trypsin. Cells presented similar immunophenotypes, adipogenic differentiation and in vitro proliferation but an osteogenic differentiation capacity up to seven times higher than ASCs isolated by collagenase. This alternative protocol is thus efficient and more cost-effective than the commonly-used methods and may represent a promising protocol for obtaining ASCs for bone tissue engineering.

引用

页码：693 / 702

页数：10

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