Single-cell resolution in high-resolution synchrotron X-ray CT imaging with gold nanoparticles

被引：21

作者：

Schueltke, Elisabeth ^{[1
]}

Menk, Ralf ^{[2
]}

Pinzer, Bernd ^{[3
]}

Astolfo, Alberto ^{[3
]}

Stampanoni, Marco ^{[3
,4
,5
]}

Arfelli, Fulvia ^{[6
,7
]}

Harsan, Laura-Adela ^{[8
]}

Nikkhah, Guido ^{[9
]}

机构：

[1] Univ Freiburg, Med Ctr, D-79106 Freiburg, Germany

[2] Sincrotrone Trieste SCpA, Trieste, Italy

[3] Paul Scherrer Inst, Swiss Light Source, Villigen, Switzerland

[4] Univ Zurich, Inst Biomed Engn, Zurich, Switzerland

[5] ETH, Zurich, Switzerland

[6] Univ Trieste, Dipartimento Fis, Trieste, Italy

[7] Ist Nazl Fis Nucl, Sez Trieste, Trieste, Italy

[8] Univ Freiburg, Med Ctr, Dept Radiol, D-79106 Freiburg, Germany

[9] Univ Klinikum Erlangen, Neurochirurg Klin, D-91054 Erlangen, Germany

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2014年 / 21卷

关键词：

animal model; brain tumour; high-resolution imaging; gold nanoparticles; X-ray CT; IN-VIVO MRI; PARTICLE-SIZE; LOADED CELLS; MICE; RADIOTHERAPY; BEAMLINE; TRACKING;

D O I：

10.1107/S1600577513029007

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Gold nanoparticles are excellent intracellular markers in X-ray imaging. Having shown previously the suitability of gold nanoparticles to detect small groups of cells with the synchrotron-based computed tomography (CT) technique both ex vivo and in vivo, it is now demonstrated that even single-cell resolution can be obtained in the brain at least ex vivo. Working in a small animal model of malignant brain tumour, the image quality obtained with different imaging modalities was compared. To generate the brain tumour, 1 x 10(5) C6 glioma cells were loaded with gold nanoparticles and implanted in the right cerebral hemisphere of an adult rat. Raw data were acquired with absorption X-ray CT followed by a local tomography technique based on synchrotron X-ray absorption yielding single-cell resolution. The reconstructed synchrotron X-ray images were compared with images obtained by small animal magnetic resonance imaging. The presence of gold nanoparticles in the tumour tissue was verified in histological sections.

引用

页码：242 / 250

页数：9

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