The Genome Sequence of 'Mycobacterium massiliense' Strain CIP 108297 Suggests the Independent Taxonomic Status of the Mycobacterium abscessus Complex at the Subspecies Level

被引：31

作者：

Cho, Yong-Joon ^{[1
]}

Yi, Hana ^{[2
,3
]}

Chun, Jongsik ^{[1
]}

Cho, Sang-Nae ^{[4
,5
]}

Daley, Charles L. ^{[6
]}

Koh, Won-Jung ^{[7
]}

Shin, Sung Jae ^{[4
,5
]}

机构：

[1] Seoul Natl Univ, Chunlab Inc, Seoul, South Korea

[2] Korea Univ, Grad Sch, Dept Publ Hlth Sci, Seoul, South Korea

[3] Korea Univ, Coll Med, Guro Hosp, Seoul 136705, South Korea

[4] Yonsei Univ, Coll Med, Dept Microbiol, Seoul, South Korea

[5] Yonsei Univ, Coll Med, Inst Immunol & Immunol Dis, Seoul, South Korea

[6] Natl Jewish Hlth, Dept Med, Div Mycobacterial & Resp Infect, Denver, CO USA

[7] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Med,Div Pulm & Crit Care Med, Seoul, South Korea

来源：

PLOS ONE | 2013年 / 8卷 / 11期

关键词：

DNA-DNA HYBRIDIZATION; SP-NOV; NONTUBERCULOUS MYCOBACTERIA; SPECIES DEFINITION; PULMONARY-DISEASE; CYSTIC-FIBROSIS; CHELONAE; BOLLETII; INFECTIONS; RESISTANCE;

D O I：

10.1371/journal.pone.0081560

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Members of the Mycabacterium abscessus complex are rapidly growing mycobacteria that are emerging as human pathogens. The M. abscassus complex was previously composed of three species, namely M. abscessus sensu strict, 'M. massiliense', and M. bolletii', In 2011, 'M. massiliense' and 'M. bolletre' were united and reclassified as a single subspecies within M. abscessus: M. abscessus subsp. bolletii. However, the placement of 'M. massiliense' Within the boundary of M. abscessus subsp. balletii remains highly controversial with regard to clinical aspects. In this study, we revisited the taxonomic status of members of the M. abscessus complex based on comparative analysis of he whole-genome sequences of 53 strains, The genome sequence of the previous type strain of 'Mycobacterium massiliense' (CIP 108297) was determined using next-generation sequencing. The genome tree based on average nucleotide identity (AN I) values supported the differentiation of M. bolletii' and M. massiliense' at the subspecies level. The genome tree also clearly illustrated that 'M. bolletil' and 'M. massiliense' form a distinct phylogenetic clade within the radiation of the M. abscessus complex. The genomic distances observed in this study suggest that the current M. abscessus subsp. bolletii taxon should be divided into two subspecies, M. abscessus subsp. massiliense subsp. nov. and M. abscessus subsp. bolletii, to correspondingly accommodate the previously known 'M. assiliense' and 'M. bolletii' strains.

引用

页数：10

共 50 条

[1] rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp nov., Mycobacterium phocaicum sp nov and Mycobacterium aubagnense sp nov. [J].