Rapid identification of dragon blood samples from Daemonorops draco, Dracaena cinnabari and Dracaena cochinchinensis by MALDI-TOF mass spectrometry

被引：16
|
作者：
Wu, Chunhung [1 ]
Cai, Xin-Qi [1 ]
Chang, Yu [2 ]
Chen, Chueh-Hsuan [1 ]
Ho, Tsung-Jung [3 ,4 ]
Lai, Shang-Chih [5 ,6 ]
Chen, Hao-Ping [2 ,4 ]
机构：
[1] Tamkang Univ, Dept Chem, New Taipei, Taiwan
[2] Tzu Chi Univ, Dept Biochem, 701,Sec 3,Zhongyang Rd, Hualien 97004, Taiwan
[3] Tzu Chi Gen Hosp, Dept Chinese Med, Hualien, Taiwan
[4] Tzu Chi Gen Hosp, Integrat Ctr Tradit Chinese & Modern Med, Hualien, Taiwan
[5] Tzu Chi Univ, Sch Postbaccalaureate Chinese Med, Hualien, Taiwan
[6] Tzu Chi Gen Hosp, Dept Pharm, Hualien, Taiwan
来源：
PHYTOCHEMICAL ANALYSIS | 2019年 / 30卷 / 06期
关键词：
dracorhodin; dragon blood; loureirin A; loureirin B; MALDI-TOF; DRACORHODIN PERCHLORATE;
D O I：
10.1002/pca.2852
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Introduction Dragon blood is a deep-red plant resin which has been used as folk medicine for more than a thousand years. It can be produced from at least four entirely different plant families: Asparagaceae, Arecaceae, Chamaesyce, and Fabaceae. Current pharmacopeia states that the only "authentic" source of dragon blood is the palm tree, Daemonorops draco. Objective The present study aims to find a high-throughput method to screen and identify the plant sources of commercial dragon blood products. Methodology A matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) based method for rapid screening of dracorhodin in commercial dragon blood samples was established in this study. Results Well-resolved peaks of dracorhodin in spectra were observed in the crude extracts of samples. Dragon blood samples from two other plant species, Dracaena cinnabari and Dracaena cochinchinensis, were also examined. Their indicator compounds, loureirin A and B, were detected in these plants. Conclusion A MALDI-TOF based method for preliminarily examination of commercial dragon blood samples is reported here. In contrast to MALDI-TOF, liquid chromatography mass spectrometry (LC-MS) is a time-consuming and costly method, not ideal for routine and large-scale screening of commercial samples.
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收藏
页码：720 / 726
页数：7
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