A reliable and sensitive enzyme immunoassay method for measuring 8-isoprostaglandin F-2 alpha: A marker for lipid peroxidation after experimental brain injury

被引：0

作者：

Hoffman, SW ^{[1
]}

Roof, RL ^{[1
]}

Stein, DG ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,INST ANIM BEHAV,BRAIN RES LAB,NEWARK,NJ 07102

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1996年 / 68卷 / 02期

关键词：

cortical contusion; brain trauma; free radical; prostaglandin; 8-isoprostaglandin F-2 alpha; (rat); CENTRAL-NERVOUS-SYSTEM; SUBARACHNOID HEMORRHAGE; MECHANISM; 21-AMINOSTEROIDS; ISCHEMIA; TRAUMA; RAT;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The objectives of this study were to determine (1) if levels of 8-isoprostaglandin F-2 alpha (8-isoPGF(2 alpha)), a non-enzymatically produced prostaglandin, were increased after cortical contusion and (2) if enzyme immunoassay (EIA) could be used to quantify 8-isoPGF(2 alpha) levels. 24 h after the contusion there was a significant rise in 8-isoPGF(2 alpha) compared to control levels. The levels returned to baseline by 72 h postinjury. The results show that this EIA method is a reliable and sensitive technique for measuring brain lipid peroxidation.

引用

页码：133 / 136

页数：4