The effect of 1,25 dihydroxyvitamin D3 on HCl/Ethanol-induced gastric injury in rats

被引:12
作者
Sahin, Hasan Huseyin [1 ]
Cumbul, Alev [2 ]
Uslu, Unal [2 ]
Yilmaz, Zeynep [3 ]
Ercan, Feriha [3 ]
Alican, Inci [1 ]
机构
[1] Marmara Univ, Sch Med, Dept Physiol, TR-34854 Istanbul, Turkey
[2] Yeditepe Univ, Sch Med, Dept Histol & Embryol, TR-34755 Istanbul, Turkey
[3] Marmara Univ, Sch Med, Dept Histol & Embryol, TR-34854 Istanbul, Turkey
关键词
1,25 dihydroxyvitamin D3; HCl/Ethanol; Gastric; Rat; KAPPA-B ACTIVATION; VITAMIN-D; NEUTROPHIL INFILTRATION; ENDOGENOUS SULFHYDRYLS; ANTIOXIDANT PROPERTIES; OXIDATIVE STRESS; MUCOSAL DEFENSE; NITRIC-OXIDE; ETHANOL; INFLAMMATION;
D O I
10.1016/j.tice.2018.03.003
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
This study evaluated ulceroprotective and antioxidant effect of 1,25 dihydroxyvitamin D3 against gastric damage in rats. Rats were treated intraperitoneally with either 1,25 dihydroxyvitamin D3 (0.25 mu g/kg) or saline for 14 days. On day-15, the non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg; subcutaneously), the inhibitor of sulfhydryl groups N-ethylmaleimide (10 mg/kg; intraperitoneally) or ATP-sensitive K+ channel blocker glibenclamide (10 mg/kg; orally) was given prior to 1,25 dihydroxyvitamin D3. Animals were euthanized at 60 min post ulcerogenic challenge (0.3 M HCl and 60% ethanol (0.2 mL; orally). Stomach and blood were collected for biochemical and histological evaluations. HCl/Ethanol group revealed severely damaged mucous and glandular epithelium with diffuse hemorrhage and inflammatory cell infiltration (microscopic score: 10.67 +/- 0.67 and ulcer index: 33.13 +/- 5.09). 1,25 dihydroxyvitamin D3 decreased the extent of damage (microscopic score: 6.80 +/- 0.02 and ulcer index: 19.00 +/- 4.34; p < 0.05), and the elevations in gastric malondialdehyde level (p < 0.001), myeloperoxidase activity (p < 0.001), nuclear factor-kappa B expression (p < 0.05), and apoptotic index (p < 0.05) following HCl/Ethanol challenge. Decreased gastric glutathione following HCl/Ethanol administration was restored by 1,25 dihydroxyvitamin D3 (p < 0.01). These findings demonstrated protection of the gastric mucosa against HCl/Ethanol-induced injury by 1,25 dihydroxyvitamin D3 via attenuation of inflammatory reaction, oxidative stress and apoptosis.
引用
收藏
页码:68 / 76
页数:9
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