Identification of Pseudomonas syringae pv. syringae causing bacterial leaf blight of Miscanthus sinensis

被引:10
作者
Choi, Okhee [1 ]
Kang, Byeongsam [2 ]
Cho, Su Kyung [2 ]
Park, Jiyeong [2 ]
Lee, Yeyeong [2 ]
Kim, Won-Il [3 ]
Marunga, Joan [4 ]
Hwang, Ingyu [4 ]
Kim, Jinwoo [1 ,2 ]
机构
[1] Gyeongsang Natl Univ, Inst Agr & Life Sci, Jinju 52828, South Korea
[2] Gyeongsang Natl Univ, Div Appl Life Sci, Jinju, South Korea
[3] Natl Inst Agr Sci, Microbial Safety Team, RDA, Wanju Gun 55365, South Korea
[4] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 08826, South Korea
基金
新加坡国家研究基金会;
关键词
Bacterial leaf blight; Miscanthus sinensis; Pseudomonas syringae pv. syringae; rpoD; 1ST REPORT;
D O I
10.1007/s41348-016-0058-4
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
In the late summer of 2015, severe leaf blight occurred on Miscanthus sinensis grown on natural riverside lands of the Han River in Seoul, South Korea. Symptoms included chlorosis and necrotic lesions on the leaves. Gram-negative, non-spore-forming, and rod-shaped Pseudomonas syringae were consistently recovered from the infected leaves. We provide ample evidence for P. syringae pv. syringae (Pss) as the pathogen, based on the results of LOPAT, Biolog, API tests, MALDI-TOF MS, and multilocus sequence analysis. Symptoms after artificial inoculation were mild compared to symptoms following natural infection, whereas the control plants were asymptomatic. Bacteria re-isolated from leaves with mild symptoms were identical to the original strain, as determined by the rpoD gene sequence. This is the first report of Pss causing bacterial leaf blight on M. sinensis.
引用
收藏
页码:97 / 100
页数:4
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