Monoclonal antibodies against Candida rugosa lipase

被引：12

作者：

Rahimi, H

Soro, S

Rughetti, A

Palocci, C

Biffoni, M

Barachini, S

Taurino, F

Cernia, E

Frati, L

Nuti, M

机构：

[1] Univ Roma La Sapienza, Dept Expt Med & Pathol, I-00161 Rome, Italy

[2] Univ Roma La Sapienza, Dept Chem, I-00161 Rome, Italy

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2004年 / 28卷 / 2-3期

关键词：

candida rugosa lipase; microbial lipase; IgG(1); isotype;

D O I：

10.1016/j.molcatb.2004.01.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG(1) isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The CRL enzyme maintained its enzymatic activity even when immunocomplexed with MoAb BF11 and VNH9. These novel reagents are of interest for their possible application as immobilization and purification systems, and during fermentation processes. (C) 2004 Elsevier B.V. All rights reserved.

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收藏

页码：71 / 74

页数：4

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