Very Fast Electrophoretic Determination of Creatinine and Uric Acid in Human Urine using a Combination of Two Capillaries with Different Inner Diameters

被引：0

作者：

Pavlicek, Vaclav ^{[1
]}

Tuma, Petr ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Med 3, Inst Biochem Cell & Mol Biol, Prague 10000 10, Czech Republic

来源：

XXXIII MODERNI ELEKTROCHEMICKE METODY | 2013年

关键词：

Capillary electrophoresis; Creatinine; Diode-array detector; Uric acid; LIQUID-CHROMATOGRAPHY; SERUM CREATININE;

D O I：

暂无

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Very fast electrophoretic methods have been developed for determination of creatinine and uric acid in human urine with diode-array detection. The separations were performed in a laboratory-made combination of two capillaries with different inner diameters. Determination of creatinine was successfully performed in 20 mM citric acid/NaOH (pH 3.0) and the determination of uric acid in 30 mM MES/NaOH (pH 6.0) as background electrolytes. The developed methods are characterized by a separation time around 10 s and limits of detection 1.4 M for both analytes. A dilution of the urine sample with water was the only step necessary before the electrophoresis analysis.

引用

页码：144 / 148

页数：5

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