Multiplex RT-PCR for Rapid Detection of Viruses Commonly Causing Diarrhea in Pediatric Patients

被引：45
|
作者：
Thongprachum, Aksara [1 ,2 ]
Khamrin, Pattara [3 ]
Ngan Thi Kim Pham [1 ]
Takanashi, Sayaka [2 ]
Okitsu, Shoko [1 ,2 ]
Shimizu, Hiroyuki [4 ]
Maneekarn, Niwat [3 ]
Hayakawa, Satoshi [1 ]
Ushijima, Hiroshi [1 ,2 ]
机构：
[1] Nihon Univ, Sch Med, Dept Pathol & Microbiol, Div Microbiol, Tokyo, Japan
[2] Univ Tokyo, Sch Int Hlth, Grad Sch Med, Dept Dev Med Sci, Tokyo, Japan
[3] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai, Thailand
[4] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan
来源：
JOURNAL OF MEDICAL VIROLOGY | 2017年 / 89卷 / 05期
基金：
日本学术振兴会;
关键词：
multiplex RT-PCR; pediatric patients; diarrheal viruses; AICHI VIRUS; ENTEROVIRUSES; ASTROVIRUS; CHILDREN; SAMPLES;
D O I：
10.1002/jmv.24711
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. (C) 2016 Wiley Periodicals, Inc.
引用
收藏
页码：818 / 824
页数：7
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