Multiplex RT-PCR for Rapid Detection of Viruses Commonly Causing Diarrhea in Pediatric Patients

被引：45

作者：

Thongprachum, Aksara ^{[1
,2
]}

Khamrin, Pattara ^{[3
]}

Ngan Thi Kim Pham ^{[1
]}

Takanashi, Sayaka ^{[2
]}

Okitsu, Shoko ^{[1
,2
]}

Shimizu, Hiroyuki ^{[4
]}

Maneekarn, Niwat ^{[3
]}

Hayakawa, Satoshi ^{[1
]}

Ushijima, Hiroshi ^{[1
,2
]}

机构：

[1] Nihon Univ, Sch Med, Dept Pathol & Microbiol, Div Microbiol, Tokyo, Japan

[2] Univ Tokyo, Sch Int Hlth, Grad Sch Med, Dept Dev Med Sci, Tokyo, Japan

[3] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai, Thailand

[4] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan

来源：

JOURNAL OF MEDICAL VIROLOGY | 2017年 / 89卷 / 05期

基金：

日本学术振兴会;

关键词：

multiplex RT-PCR; pediatric patients; diarrheal viruses; AICHI VIRUS; ENTEROVIRUSES; ASTROVIRUS; CHILDREN; SAMPLES;

D O I：

10.1002/jmv.24711

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. (C) 2016 Wiley Periodicals, Inc.

引用

页码：818 / 824

页数：7

共 50 条

[21] Establishment of a multiplex RT-PCR assay for the rapid detection of fish cytokines
Kono, Tomoya
Takayama, Hiroaki
Nagamine, Ryusuke
Korenaga, Hiroki
Sakai, Masahiro
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2013, 151 (1-2) : 90 - 101
[22] Multiplex RT-PCR for detecting nineteen respiratory viruses
Mahony, J.
Chong, S.
Merante, F.
Luinstra, K.
Sinha, T.
Petrich, A.
Smieja, M.
Lisle, C.
Yaghoubian, S.
Janeczko, R.
JOURNAL OF CLINICAL VIROLOGY, 2006, 36 : S9 - S9
[23] A multiplex RT-PCR assay for rapid and sensitive detection of cytokines in fish
Kono, T.
Takayama, H.
Nagamine, R.
Korenaga, H.
Sakai, M.
CYTOKINE, 2012, 59 (03) : 528 - 529
[24] Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR
Ishikawa, K
Sekiguchi, H
Ogino, T
Suzuki, S
JOURNAL OF VIROLOGICAL METHODS, 1997, 69 (1-2) : 191 - 195
[25] A Multiplex RT-PCR for the Detection of Three Viruses and One Viroid Infecting Hemp
Grunwald, Derrick J.
MacWilliams, Jacob
Hackenberg, Laine
Stroschein, Sydney
Rioux, Renee
Nachappa, Punya
Ellison, Shelby
PHYTOFRONTIERS, 2024,
[26] Multiplex RT-PCR assay for simultaneous detection of six viruses of penaeid shrimp
Khawsak, Paisarn
Deesukon, Warin
Chaivisuthangkura, Parin
Sukhumsirichart, Wasana
MOLECULAR AND CELLULAR PROBES, 2008, 22 (03) : 177 - 183
[27] Multiplex RT-PCR Assay for the Simultaneous Detection and Identification of Five Sugarcane Viruses
Feng, Xiao-Yan
Wang, Wen-Zhi
Shen, Lin-Bo
Wang, Jun-Gang
Xiong, Guo-Ru
Feng, Cui-Lian
Zhao, Ting-Ting
Zhang, Shu-Zhen
SUGAR TECH, 2020, 22 (04) : 662 - 670
[28] Simultaneous detection of enteric viruses by multiplex real-time RT-PCR
Beuret, C
JOURNAL OF VIROLOGICAL METHODS, 2004, 115 (01) : 1 - 8
[29] Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR
Ge, Beibei
Li, Qian
Liu, Guojie
Lu, Meiguang
Li, Shifang
Wang, Hongqing
ARCHIVES OF VIROLOGY, 2013, 158 (06) : 1181 - 1187
[30] Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR
Beibei Ge
Qian Li
Guojie Liu
Meiguang Lu
Shifang Li
Hongqing Wang
Archives of Virology, 2013, 158 : 1181 - 1187

← 1 2 3 4 5 →