Inhibitory effect of GB-2a (I3-naringenin-II8-eriodictyol) on melanogenesis

被引：15

作者：

Campos, Patricia Mazureki ^{[1
]}

Prudente, Arthur Silveira ^{[2
]}

da Silva Horinouchi, Cintia Delai ^{[2
]}

Cechinel-Filho, Valdir ^{[3
]}

Favero, Giovani Marino ^{[4
]}

Cabrini, Daniela Almeida ^{[2
]}

Otuki, Michel Fleith ^{[2
]}

机构：

[1] Univ Fed Parana, Dept Pharmaceut Sci, BR-80210170 Curitiba, Parana, Brazil

[2] Univ Fed Parana, Dept Pharmacol, BR-81530900 Curitiba, Parana, Brazil

[3] Univ Vale Itajai, Invest Ctr Pharmaceut Chem, BR-88302202 Itajai, SC, Brazil

[4] Univ Estadual Ponta Grossa, Dept Biol Sci, BR-84030900 Ponta Grossa, PR, Brazil

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2015年 / 174卷

关键词：

I3-Naringenin-II8-eriodictyol; Tyrosinase inhibition; Melanin; Garcinia gardneriana; Hyperpigmentation; Biflavonoid; HYDROALCOHOLIC EXTRACT; GENUS-RHEEDIA; TYROSINASE; BIFLAVONOIDS; SKIN; HYPERPIGMENTATION; PIGMENTATION; XANTHONES; KURARINOL;

D O I：

10.1016/j.jep.2015.08.015

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacology relevance: GB-2a is a I3-naringenin-II8-eriodictyol compound isolated from Garcinia gardneriana (Planchon & Triana) Zappi, a plant used in folk medicine for the treatment of skin disorders. Aim of study: In the search for new depigmenting agents, this study was carried out to investigate the in vitro effects of GB-2a isolated from G. gardneriana (Planchon & Triana) Zappi in B16F10 melanoma cells. Materials and methods: The effects of GB-2a were evaluated through determination of melanin biosynthesis in B16F10 melanoma cells in comparison with the reference drug kojic acid (500 mu M). In parallel, the GB-2a effect was assessed in a cell viability assay. Mushroom tyrosinase activity assays were conducted to verify the effect of this enzyme. In order to ascertain the nature of enzyme inhibition on tyrosinase, kinetics analysis of the GB-2a was performed with c-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) substrates. Results: The results showed that GB-2a biflavonoid significantly inhibited the melanin content, without reducing cell viability. GB-2a also showed a strong antityrosinase activity in the mushroom tyrosinase assay. GB-2a inhibited the tyrosinase activity, exerting a mixed inhibition. For the c-tyrosine substrate the inhibition was in non-competitive mode and for L-DOPA it was in uncompetitive mode. Conclusion: GB-2a biflavonoid promoted inhibition on tyrosinase activity and reduced melanin biosynthesis in B16F10 cells, which suggests great potential for medical and cosmetic uses as a depigmenting agent. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

引用

页码：224 / 229

页数：6

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