Production of haploids and doubled haploids of the amphiploids Aegilops variabilis x Secale cereale

Anther culture was used to produce haploid plants from Aegilops vatiabilis ssp, euvariabilis x Secale cereale (inbred line) amphiploids (2n=42) and their parental forms. The anthers were cultured on modified induction media - C-17 and PII. Androgenetic embryos were obtained only from amphiploid plants and the frequency depended on genotypes. On C-17 medium 20.0 - 65.2 and on PII medium 1.4 - 15.7 androgenetic embryos /100 anthers were produced. The embryos were transferred to a regeneration medium 190-2 and 0.1 - 13.4 of green plants 1100 androgenetic embryos were obtained. The haploid (n=21) chromosome number was found in all plants, Doubled haploids were produced by haploid colchicine treatment.