Quantitative Determination of Triterpenoids and Formononetin in Rhizomes of Black Cohosh (Actaea racemosa) and Dietary Supplements by Using UPLC-UV/ELS Detection and Identification by UPLC-MS

被引：31

作者：

Avula, Bharathi ^{[1
]}

Wang, Yan-Hong ^{[1
]}

Smillie, Troy J. ^{[1
]}

Khan, Ikhlas A. ^{[1
,2
]}

机构：

[1] Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA

来源：

PLANTA MEDICA | 2009年 / 75卷 / 04期

关键词：

Actaea racemosa; Ranunculaceae; triterpenoids; formononetin; dietary supplements; UPLC-ELSD/MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; EVAPORATIVE LIGHT-SCATTERING; CIMICIFUGA-RACEMOSA; GLYCOSIDES; PRODUCTS;

D O I：

10.1055/s-0028-1088384

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A UPLC-UV/ELSD method has been developed for analysis of major triterpenoids and formononetin in Actaea racemosa L. (family Ranunculaceae) samples. The best results were obtained with an Acquity UPLC BEH C18 (100 mm x 2.1 mm, i.d., 1 mu m) column system using gradient elution with a mobile phase consisting of water and acetonitrile:methanol (7:3) at a constant flow rate of 0.3 mL/min. Owing to their low UV absorption, the triterpene saponins were detected by evaporative light scattering. Within 5.5 minutes, three main triterpenoid glycosides [cimiracemoside A, 23-epi-26-deoxyactein, and actein] and an isoflavonoid, formononetin, could be separated, with

引用

页码：381 / 386

页数：6

共 12 条

[1] Chemical fingerprinting of Actaea racemosa (black cohosh) and its comparison study with closely related Actaea species (A. pachypoda, A. podocarpa, A. rubra) by HPLC [J].

Avula, Bharathi ;

Ali, Zulfiqar ;

Khan, Ikhlas A. .

CHROMATOGRAPHIA, 2007, 66 (9-10) :757-762

[2]

Bedir E, 2001, PHARMAZIE, V56, P268

[3]

FREEBURG ED, 2007, 233 ACS NAT M CHIC, P25

[4] Separation of Cimicifuga racemosa triterpene glycosides by reversed phase high performance liquid chromatography and evaporative light scattering detection [J].

Ganzera, M ;

Bedir, E ;

Khan, IA .

CHROMATOGRAPHIA, 2000, 52 (5-6) :301-304

[5] Direct analysis and identification of triterpene glycosides by LC/MS in black cohosh, Cimicifuga racemosa, and in several commercially available black cohosh products [J].

He, K ;

Zheng, BL ;

Kim, CH ;

Rogers, L ;

Zheng, QY .

PLANTA MEDICA, 2000, 66 (07) :635-640

[6] Cimicifuga species identification by high performance liquid chromatography-photodiode array/mass spectrometric/evaporative light scattering detection for quality control of black cohosh products [J].

He, K ;

Pauli, GF ;

Zheng, B ;

Wang, HK ;

Bai, NS ;

Peng, TS ;

Roller, M ;

Zheng, QY .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1112 (1-2) :241-254

[7] STUDIES ON THE ENDOCRINE EFFECTS OF THE CONTENTS OF CIMICIFUGA-RACEMOSA .2. INVITRO BINDING OF COMPOUNDS TO ESTROGEN-RECEPTORS [J].

JARRY, H ;

HARNISCHFEGER, G ;

DUKER, E .

PLANTA MEDICA, 1985, 51 (04) :316-319

[8] Analysis of formononetin from black cohosh (Actaea racemosa) [J].

Jiang, B. ;

Kronenberg, F. ;

Balick, M. J. ;

Kennelly, E. J. .

PHYTOMEDICINE, 2006, 13 (07) :477-486

[9] Evaluation of the botanical authenticity and phytochemical profile of black cohosh products by high-performance liquid chromatography with selected ion monitoring liquid chromatography-mass spectrometry [J].

Jiang, Bei ;

Kronenberg, Fredi ;

Qiu, Ming-Hua ;

Kennelly, Edward J. .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (09) :3242-3253

[10] Analysis of thirteen populations of Black Cohosh for formononetin [J].

Kennelly, EJ ;

Baggett, S ;

Nuntanakorn, P ;

Ososki, AL ;

Mori, SA ;

Duke, J ;

Coleton, M ;

Kronenberg, F .

PHYTOMEDICINE, 2002, 9 (05) :461-467

← 1 2 →