Ovicidal and larvicidal activity of crude extracts of Maesa lanceolata and Plectranthus punctatus against Haemonchus contortus

被引:30
作者
Tadesse, Dereje [2 ]
Eguale, Tadesse [1 ]
Giday, Mirutse [3 ]
Mussa, Abiy [2 ]
机构
[1] Natl Anim Hlth Diagnost & Invest Ctr, Minist Agr & Rural Dev, Sebeta, Ethiopia
[2] Haramaya Univ, Fac Vet Med, Dire Dawa, Ethiopia
[3] Univ Addis Ababa, Aklilu Lemma Inst Pathobiol, Addis Ababa, Ethiopia
关键词
Haemonchus contortus; Plectranthus punctatus; Maesa lanceolata; Egg hatch assay; Larval development test; IN-VITRO; ANTHELMINTIC ACTIVITY; MEDICINAL-PLANTS; HELMINTH CONTROL; NEMATODE; RUMINANTS;
D O I
10.1016/j.jep.2009.01.014
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The widespread development of anthelmintic resistance and high cost of the conventional anthelmintic drugs, has limited the control of gastrointestinal nematode parasites of sheep and goats and hence led to evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in vitro ovicidal and larvicidal activity of the leaves and fruits of the aqueous and hydro-alcoholic extracts of Maesa lanceolata and aerial parts of Plectranthus punctatus were evaluated on the egg and larvae of Haemonchus contortus using egg hatch assay and larval development test. All extracts of plants tested have shown complete inhibition of egg hatching at or below 1 mg/ml. ED50 for egg hatch inhibition ranged from 0.11 to 0.29 mg/ml, for both the aqueous and hydro-alcoholic extracts of Plectranthus punctatus and Maesa lanceolata. All extracts have shown dose dependent inhibition of larval development with variable results. The complete inhibition (100%) at the maximum concentration tested (50 mg/ml) was obtained only for hydro-alcoholic extract of the fruits of Maesa lanceolata and the lowest inhibition (50.33%) was recorded for the hydro-alcoholic extract of the leaves of the same plant. The overall findings of the present study has shown that Plectranthus punctatus and Maesa lanceolata contain possible anthelmintic compounds and further evaluation of different extracts and fractions of these plants should be carried out. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:240 / 244
页数:5
相关论文
共 34 条
[1]  
ABDU PA, 2000, ETHNOVETERINARY PRAC
[2]  
ABEBE D, 2003, ILLUSTRATED CHECKLIS, P39
[3]   In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity [J].
Alawa, CBI ;
Adamu, AM ;
Gefu, JO ;
Ajanusi, OJ ;
Abdu, PA ;
Chiezey, NP ;
Alawa, JN ;
Bowman, DD .
VETERINARY PARASITOLOGY, 2003, 113 (01) :73-81
[4]   Sustainable production of root and tuber crops (potato, sweet potato, indigenous potato, cassava) in southern Africa [J].
Allemann, J ;
Laurie, SM ;
Thiart, S ;
Vorster, HJ .
SOUTH AFRICAN JOURNAL OF BOTANY, 2004, 70 (01) :60-66
[5]  
[Anonymous], ETHIOP VET J
[6]   Ethnobotanical study of some Ghanaian anti-malarial plants [J].
Asase, A ;
Oteng-Yeboah, AA ;
Odamtten, GT ;
Simmonds, MSJ .
JOURNAL OF ETHNOPHARMACOLOGY, 2005, 99 (02) :273-279
[7]   Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn.: extracts on Haemonchus contortus [J].
Assis, LM ;
Bevilaqua, CML ;
Morais, SM ;
Vieira, LS ;
Costa, CTC ;
Souza, JAL .
VETERINARY PARASITOLOGY, 2003, 117 (1-2) :43-49
[8]   Irradiated vaccines for helminth control in livestock [J].
Bain, RK .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1999, 29 (01) :185-191
[9]   Biological control of nematode parasites of small ruminants in Malaysia using the nematophagous fungus Duddingtonia flagrans [J].
Chandrawathani, P ;
Jamnah, O ;
Waller, PJ ;
Larsen, M ;
Gillespie, AT ;
Zahari, WM .
VETERINARY PARASITOLOGY, 2003, 117 (03) :173-183
[10]   WORLD-ASSOCIATION-FOR-THE-ADVANCEMENT-OF-VETERINARY-PARASITOLOGY (WAAVP) METHODS FOR THE DETECTION OF ANTHELMINTIC RESISTANCE IN NEMATODES OF VETERINARY IMPORTANCE [J].
COLES, GC ;
BAUER, C ;
BORGSTEEDE, FHM ;
GEERTS, S ;
KLEI, TR ;
TAYLOR, MA ;
WALLER, PJ .
VETERINARY PARASITOLOGY, 1992, 44 (1-2) :35-44