Environmental friendly cold-mechanical/sonic enzymatic assisted extraction of genipin from genipap (Genipa americana)

被引：28

作者：

Mayela Ramos-de-la-Pena, Ana ^{[1
]}

Renard, Catherine M. G. C. ^{[2
]}

Wicker, Louise ^{[3
]}

Montanez, Julio C. ^{[1
]}

Alfonso Garcia-Cerda, Luis ^{[4
]}

Carlos Contreras-Esquivel, Juan ^{[1
,5
]}

机构：

[1] Univ Autonoma Coahuila, Sch Chem, Food Res Dept, Lab Appl Glycobiotechnol, Saltillo 25000, Coahuila, Mexico

[2] Univ Avignon, INRA, Secur & Qual Prod Origine Vegetale UMR408, F-84000 Avignon, France

[3] Univ Gerogia, Dept Food Sci & Technol, Athens, GA 30602 USA

[4] Ctr Invest Quim Aplicada, Dept Mat Avanzados, Saltillo 25250, Coahuila, Mexico

[5] Coyotefoods Biopolymer & Biotechnol Co, Ctr Res & Dev, Saltillo 25000, Coahuila, Mexico

来源：

ULTRASONICS SONOCHEMISTRY | 2014年 / 21卷 / 01期

关键词：

Ultrasound; Pectin; Pectinesterase; Protein; Polyelectrolyte complex; COLORIMETRIC DETERMINATION; GENIPOSIDE; ULTRASOUND;

D O I：

10.1016/j.ultsonch.2013.06.008

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

An efficient cold-mechanical/sonic-assisted extraction technique was developed for extraction of genipin from genipap (Genipa americana) peel. Ultrasound assisted extraction (285 W, 24 kHz) was performed at 5, 10 and 15 degrees C for 5, 10 and 15 min. After cold-extraction, genipin was separated from pectin and proteins by aid of fungal pectinesterase. The maximum yield of non-cross-linked genipin was 7.85 +/- 0.33 mg/g, at 10 degrees C for 15 min by means of ultrasound extraction. The protein amount in extracts decreased in all samples. If mechanical process is combined with ultrasound assisted extraction the yield is increased by 8 times after the pectinesterase-assisted polyelectrolyte complex formation between pectic polysaccharides and proteins, avoiding the typical cross-linking of genipin. This novel process is viable to obtain non-cross-linked genipin, to be used as a natural colorant and cross-linker in the food and biotechnological industries. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：43 / 49

页数：7

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