Hydrodistilled volatile oil obtained from Artemisia integrifolia, cultivated in Greater Khingan Mountains, was analyzed by gas chromatography and gas chromatography-mass. Sixty-one compounds were identified, representing 98.86 % of the total oil. The major constituents of essential oil were oxygenated monoterpenes, camphor (12.65 %), artemisia ketone (11.78 %), eucalyptol (6.28 %) and artemisia alcohol (6.5 6%). Antimicrobial potential of oil against different bacterial strains (Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus), yeast strains (Hansenula anomala and Saccharomy cescerevisiae) and molds (Aspergillus niger, Chaetomium globosum, Mucor racemosus and Monascus anka) was determined by disc diffusion method and broth micro dilution method, respectively. The oil (1000 mu g/disc) exhibited promising antibacterial effect as a diameter of zones of inhibition (20.5-26.6 mm). MIC values of oil were ranged from 62.5 to 250 mu g/mL.