Activity- and development-dependent down-regulation of TARPγ8 and GluA1 in cultured rat hippocampal neurons

被引:3
作者
Wang, Jian-gang [1 ,2 ]
Wang, Ya-li [1 ,3 ]
Xu, Fang [1 ,3 ]
Zhao, Jing-xi [1 ,3 ]
Zhou, Si-yuan [1 ,3 ]
Yu, Yi [1 ,3 ]
Chazot, Paul L. [4 ]
Wang, Xiao-fang [1 ]
Lu, Cheng-biao [1 ,3 ]
机构
[1] Xinxiang Med Univ, Key Lab Brain Res Henan Prov, Xinxiang 453003, Peoples R China
[2] Xinxiang Med Univ, Dept Pathophysiol, Xinxiang 453003, Peoples R China
[3] Xinxiang Med Univ, Dept Physiol & Neurobiol, Xinxiang 453003, Peoples R China
[4] Univ Durham, Sch Biol & Biomed Sci, Durham DH1 3LE, England
基金
中国国家自然科学基金;
关键词
AMPA receptor; cultured hippocampal neurons; TARP gamma 8; GluA1; calpain; calpeptin; activity-dependent; development-dependent; AMPA RECEPTOR SUBUNITS; TARP GAMMA-8; STARGAZIN; EXPRESSION; APOPTOSIS; PROTECTS; PROTEASE; NECROSIS; CALCIUM; BINDING;
D O I
10.1038/aps.2015.112
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Aim: Transmembrane AMPA receptor regulatory proteins (TARPs) regulate the trafficking and expression of AMPA receptors that are essential for the fast excitatory synaptic transmission and plasticity in the brain. This study aimed to investigate the activity-dependent regulation of TARP gamma 8 in cultured rat hippocampal neurons. Methods: Rat hippocampal neurons cultured for 7-8 DIV or 17-18 DIV were exposed to the AMPA receptor agonist AMPA at a non-toxic concentration (100 mu mol/L) for 4 h. The protein levels of TARP gamma 8 and AMPA receptor subunits (GluA1 and GluA2) were measured using Western blotting analysis. AMPA-induced currents were recorded in the neurons using a whole-cell recording method. Results: Four-hour exposure to AMPA significantly decreased the protein levels of TARP gamma 8 and GluA1 in the neurons at 17-18 DIV, but did not change the protein level of TARP gamma 8 in the neurons cultured at 7-8 DIV. AMPA-induced down-regulation of TARP gamma 8 and GluA1 was largely blocked by the calpain inhibitor calpeptin (50 mu mol/L), but not affected by the caspase inhibitor zVAD (50 mu mol/L). Four-hour exposure to AMPA significantly decreased AMPA-induced currents in the neurons at 17-18 DIV, which was blocked by co-exposure to calpeptin (50 mu mol/L). Conclusion: The down-regulation of TARP gamma 8 and GluA1 protein levels and AMPA-induced currents in cultured rat hippocampal neurons is activity-and development-dependent, and mediated by endogenous calpain.
引用
收藏
页码:303 / 311
页数:9
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共 40 条
  • [11] Ceftriaxone modulates uptake activity of glial glutamate transporter-1 against global brain ischemia in rats
    Hu, Yu-Yan
    Xu, Jing
    Zhang, Min
    Wang, Dan
    Li, Li
    Li, Wen-Bin
    [J]. JOURNAL OF NEUROCHEMISTRY, 2015, 132 (02) : 194 - 205
  • [12] Age-related loss of the AMPA receptor subunits GluR2/3 in the human nucleus basalis of Meynert
    Ikonomovic, MD
    Nocera, R
    Mizukami, K
    Armstrong, DM
    [J]. EXPERIMENTAL NEUROLOGY, 2000, 166 (02) : 363 - 375
  • [13] Stargazin (TARP γ-2) Is Required for Compartment-Specific AMPA Receptor Trafficking and Synaptic Plasticity in Cerebellar Stellate Cells
    Jackson, Alexander C.
    Nicoll, Roger A.
    [J]. JOURNAL OF NEUROSCIENCE, 2011, 31 (11) : 3939 - 3952
  • [14] Induction of a caspase-3-like activity by calcium in normal cytosolic extracts triggers nuclear apoptosis in a cell-free system
    Juin, P
    Pelletier, M
    Oliver, L
    Tremblais, K
    Grégoire, P
    Meflah, K
    Vallette, FM
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (28) : 17559 - 17564
  • [15] Age Peculiarities of the Calpain/Calpastatin Cerebral System in Rats: Relation to the Hypothesis of Brain Aging
    Kastrikina, T. F.
    Stel'makh, L. N.
    Malysheva, M. K.
    [J]. NEUROPHYSIOLOGY, 2009, 41 (02) : 95 - 99
  • [16] AMPA receptor subunit-specific regulation by a distinct family of type II TARPs
    Kato, Akihiko S.
    Siuda, Edward R.
    Nisenbaum, Eric S.
    Bredt, David S.
    [J]. NEURON, 2008, 59 (06) : 986 - 996
  • [17] Hippocampal AMPA Receptor Gating Controlled by Both TARP and Cornichon Proteins
    Kato, Akihiko S.
    Gill, Martin B.
    Ho, Michelle T.
    Yu, Hong
    Tu, Yuan
    Siuda, Edward R.
    Wang, He
    Qian, Yue-Wei
    Nisenbaum, Eric S.
    Tomita, Susumu
    Bredt, David S.
    [J]. NEURON, 2010, 68 (06) : 1082 - 1096
  • [18] TARPs differentially decorate AMPA receptors to specify neuropharmacology
    Kato, Akihiko S.
    Gill, Martin B.
    Yu, Hong
    Nisenbaum, Eric S.
    Bredt, David S.
    [J]. TRENDS IN NEUROSCIENCES, 2010, 33 (05) : 241 - 248
  • [19] Neuronal loss in primary long-term cortical culture involves neurodegeneration-like cell death via calpain and p35 processing, but not developmental apoptosis or aging
    Kim, Min-Ju
    Oh, Soo-Jin
    Park, Seong-Hoon
    Kang, Hong-Jun
    Won, Moo Ho
    Kang, Tae-Cheon
    Park, Jae-Bong
    Kim, Jong-Il
    Kim, Jaebong
    Lee, Jae-Yong
    [J]. EXPERIMENTAL AND MOLECULAR MEDICINE, 2007, 39 (01) : 14 - 26
  • [20] Evidence that caspase-1 is a negative regulator of AMPA receptor-mediated long-term potentiation at hippocampal synapses
    Lu, CB
    Wang, Y
    Furukawa, K
    Fu, WM
    Ouyang, X
    Mattson, MP
    [J]. JOURNAL OF NEUROCHEMISTRY, 2006, 97 (04) : 1104 - 1110