Evidence for electro-chemical interactions between multi-walled carbon nanotubes and human macrophages

被引：20

作者：

Fiorito, Silvana ^{[1
]}

Monthioux, Marc ^{[2
]}

Psaila, Rossana ^{[1
]}

Pierimarchi, Pasquale ^{[1
]}

Zonfrillo, Manuela ^{[1
]}

D'Emilia, Enrico ^{[1
]}

Grimaldi, Settimio ^{[1
]}

Lisi, Antonella ^{[1
]}

Beguin, Francois ^{[3
]}

Almairac, Robert ^{[4
]}

Noe, Laure ^{[2
]}

Serafino, Annalucia ^{[1
]}

机构：

[1] CNR, Inst Neurobiol & Mol Med, I-00133 Rome, Italy

[2] CNRS, CEMES, UPR 8011, F-31055 Toulouse, France

[3] Univ Orleans, CNRS, CRMD, F-45071 Orleans, France

[4] Univ Montpellier 2, LCVN, CNRS, F-34095 Montpellier, France

来源：

CARBON | 2009年 / 47卷 / 12期

关键词：

NEURONAL GROWTH; CELLS; STIMULATION; KERATINOCYTES; ACTIVATION; MECHANISM; TOXICITY;

D O I：

10.1016/j.carbon.2009.06.023

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Carbon multi-walled nanotubes (MWCNTs) may have several dangerous effects on different cell systems, but the mechanisms responsible for their cytotoxicity are not well known yet. At present, very little is known about the electrical interactions between nanomaterials and cells. We aimed to verify whether MWCNT electrical properties could affect the so called "charge-sensitive" cell parameters, interacting with cellular electrical activity. Human macrophages were challenged with two fully characterised MWCNT samples, one tested as-prepared (MWCNT), the other one purified (by annealing at 2400 degrees C) and better electro-conductive (a-MWCNT). Our findings show that a-MWCNTs are less cytotoxic but possess a higher inflammatory potential, as compared to MWCNTs. Moreover, only annealed and better conductive MWCNTs affect significantly the mitochondrial membrane polarity, the intracellular pH and the reorganisation of cytoskeleton actin filaments, cell functions strictly dependent on electro-chemical mechanisms. Based on our results, there is evidence for electro-chemical interactions taking place between cell membranes and electro-conductive MWCNTs. Such a specific behaviour could have wide-range applications in the biomedical field, not only concerning those cellular systems (neuronal and bone cells) sensitive to electrical stimuli, but also other cell systems. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2789 / 2804

页数：16

共 29 条

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