Synthesis and living cell imaging of a novel fluorescent sensor for selective cupric detection

被引:5
作者
Luo, Qianping [1 ]
Bandi, Koteswara Rao [2 ]
Dong, Yanqiu [1 ]
Bao, Hongli [2 ]
Li, Daliang [1 ,3 ]
Chen, Qi [1 ]
机构
[1] Fujian Normal Univ, Coll Life Sci, Biomed Res Ctr South China, Key Lab Innate Immune Biol Fujian Prov, 1 Keji Rd, Fuzhou 350117, Fujian, Peoples R China
[2] Chinese Acad Sci, Fujian Inst Res Struct Matter, Key Lab Coal Ethylene Glycol & Its Related Techno, 155 Yangqiao Rd West, Fuzhou 350002, Fujian, Peoples R China
[3] Fujian Normal Univ, Ctr Engn Technol Res Microalgae Germplasm Improve, Southern Inst Oceanog, Publ Serv Platform Industrializat Dev Technol Mar, Fuzhou 350117, Fujian, Peoples R China
关键词
Fluorescent spectroscopy; Cu(II); Absorption spectroscopy; Living cell imaging; COPPER COORDINATION; METAL-IONS; ALZHEIMERS; PARKINSONS; IRON; CHEMOSENSORS; CHEMISTRY; PROBES; ZINC;
D O I
10.1016/j.saa.2019.02.013
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
Copper is an important element indispensable for human life and health. Many copper-determining probes have been created for exploring its functional behavior in various cell types but few of them contains both fluorescent and colorimetric characters. In the present study, we developed a set of copper probes by synthesizing several novel thiophene-based Schiff bases in order to make a suitable sensor for quantifying and imaging copper in living cells. We find that the ligand FS-1 has a splendid selectivity and affinity toward Cu2+ among the common divalent metal ions. Living cell imaging show that FS-1 has a robust and repetitive fluorescence response in the presence of Cu2+ only in the cytosolic space of Hepg2 cell and not in the other cells examined. These data suggest that we have developed a new copper probe that can be used as a Cu2+ fluorescent and colorimetric sensor for in vivo and in vitro copper studies. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:146 / 151
页数:6
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