Mitogen-activated protein kinases and phosphatidylinositol 3-kinase are involved in Prevotella intermedia-induced proinflammatory cytokines expression in human periodontal ligament cells

被引:28
作者
Guan, Su-Min [1 ,2 ]
Zhang, Ming [3 ]
He, Jian-Jun [3 ]
Wu, Jun-Zheng [1 ]
机构
[1] Fourth Mil Med Univ, Dept Oral Biol, Sch Stomatol, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Dept Prevent Dent, Sch Stomatol, Xian 710032, Peoples R China
[3] Fourth Mil Med Univ, Dept Res Adm, Sch Stomatol, Xian 710032, Peoples R China
关键词
Prevotella intermedia; Cytokines; Mitogen-activated protein kinases; Periodontal ligament cells; Phosphatidylinositol; 3-kinase; COLONY-STIMULATING FACTOR; HUMAN GINGIVAL FIBROBLASTS; BLACK-PIGMENTED BACTERIA; PORPHYROMONAS-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; EPITHELIAL-CELLS; BONE-RESORPTION; KAPPA-B; INTERLEUKIN-6; OSTEOCLASTOGENESIS;
D O I
10.1016/j.bbrc.2009.06.058
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chronic periodontitis is ail inflammatory disease affecting periodontal connective tissues and alveolar bone. Proinflammatory mediators induced by periodontal pathogens play vital roles in the initiation and progression of the disease. In this study, we examined whether Prevotella intermedia induces proinflammatory cytokines expression in human periodontal ligament cells (hPDLs). The mRNA expression and protein production were determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbant assay (ELISA) respectively. P. intermedia treatment dose- and time-dependently increased IL-6, IL-8 and M-CSF, but not IL-I beta and TNF alpha mRNA expression and protein secretion. Preincubation of hPDLs with extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), p38 kinase and phosphatidylinositol 3-kinase (PI3K) inhibitors PD98059, SP600125, SB203580 and LY294002 resulted in significant reduction in P. intermedia-induced IL-6, IL-8 and M-CSF expression. Blocking the synthesis of prostaglandin E-2 (PGE(2)) by indomethacin also abolished the stimulatory effects of P. intermedia oil cytokines expression. Our results indicate that P. intermedia induces proinflammatory cytokines through MAPKs and PI3K signaling pathways, and PGE2 is involved in the P. intermedia-induced proinflammatory cytokines upregulation. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:471 / 476
页数:6
相关论文
共 31 条
[1]   Molecular and cell biology of the gingiva [J].
Bartold, PM ;
Walsh, LJ ;
Narayanan, S .
PERIODONTOLOGY 2000, 2000, 24 :28-55
[2]   Cytokine responses of human gingival fibroblasts to Actinobacillus actinomycetemcomitans cytolethal distending toxin [J].
Belibasakis, GN ;
Johansson, A ;
Wang, Y ;
Chen, C ;
Lagergård, T ;
Kalfas, S ;
Lerner, UH .
CYTOKINE, 2005, 30 (02) :56-63
[3]   Interleukin-8 stimulation of osteoclastogenesis and bone resorption is a mechanism for the increased osteolysis of metastatic bone disease [J].
Bendre, MS ;
Montague, DC ;
Peery, T ;
Akel, NS ;
Gaddy, D ;
Suva, LJ .
BONE, 2003, 33 (01) :28-37
[4]  
Blix IJS, 1999, EUR J ORAL SCI, V107, P14
[5]   Porphyromonas gingivalis-induced inflammatory mediator profile in an ex vivo human whole blood model [J].
Bodet, C ;
Chandad, F ;
Grenier, D .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2006, 143 (01) :50-57
[6]   Prostaglandin E(2) potentiates interleukin-1 beta induced interleukin-6 production by human gingival fibroblasts [J].
Czuszak, CA ;
Sutherland, DE ;
Billman, MA ;
Stein, SH .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1996, 23 (07) :635-640
[7]   Invasion of human oral epithelial cells by Prevotella intermedia [J].
Dorn, BR ;
Leung, KP ;
Progulske-Fox, A .
INFECTION AND IMMUNITY, 1998, 66 (12) :6054-6057
[8]   Differential activation of human gingival epithelial cells and monocytes by Porphyromonas gingivalis fimbriae [J].
Eskan, Mehmet A. ;
Hajishengallis, George ;
Kinane, Denis F. .
INFECTION AND IMMUNITY, 2007, 75 (02) :892-898
[9]   MACROPHAGE-COLONY-STIMULATING FACTOR STIMULATES SURVIVAL AND CHEMOTACTIC BEHAVIOR IN ISOLATED OSTEOCLASTS [J].
FULLER, K ;
OWENS, JM ;
JAGGER, CJ ;
WILSON, A ;
MOSS, R ;
CHAMBERS, TJ .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 178 (05) :1733-1744
[10]  
Haffajee A D, 1994, Periodontol 2000, V5, P78, DOI 10.1111/j.1600-0757.1994.tb00020.x