Biolistic-mediated transient gene expression in shoot apical meristems of the prickly-pear (Opuntia ficus-indica)

被引：7

作者：

Llamoca-Zárate, RM

Ponte, LFA

Landsmann, J

Campos, FAP

机构：

[1] Univ Fed Ceara, Dept Biochem & Mol Biol, BR-60001970 Fortaleza, Ceara, Brazil

[2] Fed Biol Res Ctr Agr & Forestry, Int Plant Virol Microbiol & Biosafety, D-38104 Braunschweig, Germany

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 1999年 / 42卷 / 03期

关键词：

Opuntia ficus-indica; prickly-pear; Cactaceae; transient gene expression; particle bombardment;

D O I：

10.1590/S1516-89131999000300005

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have demonstrated the transient expression of the GUS gene in cells of the meristematic apical dome of Opuntia ficus-indica, DNA delivery into the cells was achieved using a biolistic PDS-1000He instrument from Bio-Rad Laboratories. The transforming DNA was coated in tungsten particles with diameter of 1.3 mu m and the distance between the flying disk and the target tissue was 7.5 cm and the shooting pressure was adjusted to 1200 psi. This is the first demonstration that the biolistic transformation system can be used to express a transgene in a member of the Cactaceae.

引用

页码：299 / 302

页数：4

共 15 条

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[2]

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[3]

Jefferson RA., 1987, PLANT MOL BIOL REP, V5, P387, DOI DOI 10.1007/BF02667740

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