R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences

被引:45
作者
Casini, Arturo [1 ,2 ]
Christodoulou, Georgia [2 ]
Freemont, Paul S. [1 ,2 ]
Baldwin, Geoff S. [1 ,2 ]
Ellis, Tom [1 ,3 ]
MacDonald, James T. [1 ,2 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Ctr Synthet Biol & Innovat, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England
[3] Univ London Imperial Coll Sci Technol & Med, Dept Bioengn, London SW7 2AZ, England
基金
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
software; synthetic biology; biologically neutral sequences; linkers; GENE-EXPRESSION; CONSTRUCTION; CIRCUITS; ELEMENTS;
D O I
10.1021/sb4001323
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
R2oDNA Designer is a web application that stochastically generates orthogonal sets of synthetic DNA sequences designed to be biologically neutral. Biologically neutral sequences may be used for directing efficient DNA assembly by overlap-directed methods, as a negative control for functional DNA, as barcodes, or potentially as spacer regions to insulate biological parts from local context. The software creates optimized sequences using a Monte Carlo simulated annealing approach followed by the elimination of sequences homologous to host genomes and commonly used biological parts. An orthogonal set is finally determined by using a network elimination algorithm. Design constraints can be defined using either a web-based graphical user interface (GUI) or uploading a file containing a set of text commands.
引用
收藏
页码:525 / 528
页数:4
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