Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons

被引：6
作者：
Akter, Masuma [1 ]
Ding, Baojin [1 ]
机构：
[1] Louisiana State Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Shreveport, LA 71130 USA
来源：
CELLS | 2022年 / 11卷 / 23期
基金：
美国国家卫生研究院;
关键词：
hiPSC; motor neurons; small molecules; transcription factors; movement disorders; PLURIPOTENT STEM-CELLS; DISEASE EXPERIMENTAL-MODELS; NEURAL REST/SCP1 PATHWAY; IN-VITRO; TRANSCRIPTION FACTORS; MIR-124; ANTAGONIZES; REGULATORY NETWORK; HUMAN FIBROBLASTS; GENE-EXPRESSION; SMALL MOLECULES;
D O I：
10.3390/cells11233796
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Generation of motor neurons (MNs) from human-induced pluripotent stem cells (hiPSCs) overcomes the limited access to human brain tissues and provides an unprecedent approach for modeling MN-related diseases. In this review, we discuss the recent progression in understanding the regulatory mechanisms of MN differentiation and their applications in the generation of MNs from hiPSCs, with a particular focus on two approaches: induction by small molecules and induction by lentiviral delivery of transcription factors. At each induction stage, different culture media and supplements, typical growth conditions and cellular morphology, and specific markers for validation of cell identity and quality control are specifically discussed. Both approaches can generate functional MNs. Currently, the major challenges in modeling neurological diseases using iPSC-derived neurons are: obtaining neurons with high purity and yield; long-term neuron culture to reach full maturation; and how to culture neurons more physiologically to maximize relevance to in vivo conditions.
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页数：25
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