Usefulness of PCR-RFLP coa gene for clonal classification of methicillin-resistant Staphylococcus aureus isolates in tertiary hospitals

被引:4
作者
Gonzalez-Dominguez, Maria [1 ]
Potel, Carmen [2 ,3 ]
Seral, Cristina [1 ,4 ]
Constenla, Lucia [3 ]
Castillo, F. Javier [1 ,4 ]
Alvarez, Maximiliano [2 ,3 ]
机构
[1] Hosp Clin Univ Lozano Blesa, Serv Microbiol, Zaragoza 50009, Spain
[2] Complexo Hosp Univ Vigo, Serv Microbiol, Vigo, Spain
[3] Univ Zaragoza, IBIV, Inst Invest Biomed Vigo, Unidad Apoyo Invest, E-50009 Zaragoza, Spain
[4] Univ Zaragoza, Fac Med, Dept Microbiol, E-50009 Zaragoza, Spain
关键词
MRSA; spa typing; PCR-RFLP; coa gene; COAGULASE GENE; EPIDEMIOLOGY; SURVEILLANCE; SPA; COWS;
D O I
10.3109/00365548.2014.929735
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
One hundred and one methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates were classified into 10 genotypes based on their polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) coa pattern. PCR-RFLP coa patterns correlated with the clonal complex (CC) with the exception of CC5, which was related to 2 patterns (B and E). The PCR-RFLP coa gene technique provides a useful preliminary method to monitor variations in MRSA populations.
引用
收藏
页码:719 / 722
页数:4
相关论文
共 20 条
[1]   Coagulase gemne variants associated with distinct populations of Staphylococcus aureus [J].
Carter, PE ;
Begbie, K ;
Thomson-Carter, FM .
EPIDEMIOLOGY AND INFECTION, 2003, 130 (02) :207-219
[2]   The changing epidemiology of Staphylococcus aureus? [J].
Chambers, HF .
EMERGING INFECTIOUS DISEASES, 2001, 7 (02) :178-182
[3]  
da Silva ER, 2005, CAN J VET RES, V69, P260
[4]  
Friedrich A W, 2008, Euro Surveill, V13
[5]   MOLECULAR TYPING OF STAPHYLOCOCCUS-AUREUS ON THE BASIS OF COAGULASE GENE POLYMORPHISMS [J].
GOH, SH ;
BYRNE, SK ;
ZHANG, JL ;
CHOW, AW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1642-1645
[6]   Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management [J].
Harmsen, D ;
Claus, H ;
Witte, W ;
Rothgänger, J ;
Claus, H ;
Turnwald, D ;
Vogel, U .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) :5442-5448
[7]   Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene [J].
Hookey, JV ;
Richardson, JF ;
Cookson, BD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) :1083-1089
[8]   NUMERICAL INDEX OF THE DISCRIMINATORY ABILITY OF TYPING SYSTEMS - AN APPLICATION OF SIMPSONS INDEX OF DIVERSITY [J].
HUNTER, PR ;
GASTON, MA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (11) :2465-2466
[9]   Dominance of EMRSA-15 and-16 among MRSA causing nosocomial bacteraemia in the UK:: analysis of isolates from the European Antimicrobial Resistance Surveillance System (EARSS) -: J Antimicrob Chemother 2001;: 48:143-144 [J].
Johnson, AP ;
Aucken, HM ;
Cavendish, S ;
Ganner, M ;
Wale, MCJ ;
Warner, M ;
Livermore, DM ;
Cookson, BD .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2001, 48 (01) :143-144
[10]   Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis [J].
Karakulska, J. ;
Pobucewicz, A. ;
Nawrotek, P. ;
Muszynska, M. ;
Furowicz, A. J. ;
Czernomysy-Furowicz, D. .
POLISH JOURNAL OF VETERINARY SCIENCES, 2011, 14 (02) :285-286