Evaluation of Insulin and Ascorbic Acid Effects on Expression of Bcl-2 Family Proteins and Caspase-3 Activity in Hippocampus of STZ-Induced Diabetic Rats

被引：27

作者：

Anarkooli, Iraj Jafari ^{[2
,3
,4
]}

Sankian, Mojtaba ^{[1
]}

Vahedi, Fatemeh ^{[5
]}

Bonakdaran, Shokoofeh

Varasteh, Abdol-Reza ^{[1
]}

Haghir, Hossein ^{[2
,3
]}

机构：

[1] Mashad Univ Med Sci, Avicenna Res Inst, Immunol Res Ctr, Immunobiochem Lab, Mashhad 9196773117, Iran

[2] Mashhad Univ Med Sci, Sch Med, Dept Anat, Mashhad, Iran

[3] Mashhad Univ Med Sci, Sch Med, Neurosci Res Ctr, Mashhad, Iran

[4] Zanjan Univ Med Sci, Dept Anat, Sch Med, Zanjan, Iran

[5] Razi Vaccine & Serum Res Inst, Dept Biotechnol, Mashhad, Iran

来源：

CELLULAR AND MOLECULAR NEUROBIOLOGY | 2009年 / 29卷 / 01期

关键词：

Diabetes; Insulin; Ascorbic acid; Hippocampus; Apoptosis; Rat-Caspase-3; Bcl-2; Bcl-x(L); Bax; GLUCOSE-INDUCED APOPTOSIS; PROGRAMMED CELL-DEATH; NEURONAL APOPTOSIS; CEREBRAL-ISCHEMIA; OXIDATIVE STRESS; IN-VIVO; BAX; ACTIVATION; MECHANISMS; PROTECTION;

D O I：

10.1007/s10571-008-9305-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Aims Effects of insulin and ascorbic acid on expression of Bcl-2 family proteins and caspase-3 activity in hippocampus of diabetic rats were evaluated in this study. Methods Diabetes was induced in Wistar male rats by streptozotocin (STZ). Six weeks after verification of diabetes, the animals were treated for 2 weeks with insulin or/and ascorbic acid in separate groups. Hippocampi of rats were removed and evaluation of Bcl-2, Bcl-x(L), and Bax proteins expression in frozen hippocampi tissues were done by SDS-PAGE electrophoresis and blotting. The Bcl-2, Bcl-x(L), and Bax proteins bands were visualized after incubation with specific antibodies using enhanced chemiluminescences method. Caspase-3 activity was determined using the caspase-3/CPP32 Fluorometric Assay Kit. Results Diabetic rats showed increase in Bax protein expression and decrease in Bcl-2 and Bcl-x(L) proteins expression. The Bax/Bcl-2 and Bax/Bcl-x(L) ratios were found higher compared with non-diabetic control group. Treatments with insulin and/or ascorbic acid were resulted in decrease in Bax protein expression and increase in Bcl-2 and Bcl-xL proteins expression. The Bcl-2/Bax and Bcl-x(L)/Bax ratios were found higher in treated groups than untreated diabetic group. Caspase-3 activity level was found higher in diabetic group compared with nondiabetic group. Treatment with insulin and ascorbic acid did downregulated caspase-3 activity. Conclusions Our data provide supportive evidence to demonstrate the antiapoptotic effects of insulin and ascorbic acid on hippocampus of STZ-induced diabetic rats.

引用

页码：133 / 140

页数：8

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