Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)

被引：0

作者：

Markovic, Z. ^{[1
]}

Preiner, D. ^{[1
]}

Stupic, D. ^{[1
]}

Andabaka, Z. ^{[1
]}

Simon, S. ^{[2
]}

Voncina, D. ^{[3
]}

Maletic, E. ^{[1
]}

Kontic, J. Karoglan ^{[1
]}

Chatelet, P. ^{[4
]}

Engelmann, F. ^{[5
]}

机构：

[1] Univ Zagreb, Fac Agr, Dept Viticulture & Enol, Zagreb 10000, Croatia

[2] Univ Zagreb, Fac Agr, Dept Plant Breeding Genet & Biometr, Zagreb 10000, Croatia

[3] Univ Zagreb, Dept Plant Pathol, Zagreb 10000, Croatia

[4] INRA, UMR AGAP, Equipe DAAV, F-34060 Montpellier, France

[5] IRD, UMR DIADE, Montpellier, France

来源：

VITIS | 2015年 / 54卷

关键词：

grapevine; cryopreservation; cryotherapy; genetic stability; GROWN SHOOT TIPS; GENETIC STABILITY; VITRIFICATION; ELIMINATION; DEHYDRATION; VIRUSES;

D O I：

暂无

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

This study aimed at testing the efficiency of a droplet-vitrification cryopreservation protocol in eliminating selected grapevine viruses. The cryopreservation protocol led to approximately 50 % recovery with cultivar 'Portan' and five international cultivars tested, but very low recovery was noted with Croatian cultivars. GFLV and GLRaV-3, two (economically important grapevine) viruses were eliminated in a high percentage (up to 100 %) of plants regenerated from cryopreserved shoot tips. Virus sanitation was observed as well in samples before liquid nitrogen exposure. Genetic stability of plants regenerated after cryopreservation was studied using AFLP markers. Polymorphic fragments were observed in non-cryopreserved and cryopreserved samples treated with PVS2 solution, the number of which increased with increasing durations of exposure to PVS2 solution.

引用

页码：247 / 251

页数：5

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