1-Ethyl-3-Methylimidazolium Ethylsulfate/Copper Catalyst for the Enhancement of Glucose Chemiluminescent Detection: Effects on Light Emission and Enzyme Activity

被引：32

作者：

Santafe, Aurelie A. -M. ^{[1
]}

Doumeche, Bastien ^{[1
]}

Blum, Loic J. ^{[1
]}

Girard-Egrot, Agnes P. ^{[1
]}

Marquette, Christophe A. ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS ICBMS 5246, Lab Genie Enzymat Membranes Biomimet & Assemblage, Inst Chim & Biochim Mol & Supramol, F-69622 Villeurbanne, France

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 06期

关键词：

FLOW-INJECTION ANALYSIS; IONIC LIQUIDS; HORSERADISH-PEROXIDASE; HYDROGEN-PEROXIDE; LUMINOL CHEMILUMINESCENCE; CAPILLARY-ELECTROPHORESIS; MECHANISM; ELECTROCHEMILUMINESCENCE; BIOSENSOR;

D O I：

10.1021/ac9026725

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The effect of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO4]) on the copper-catalyzed luminol chemiluminescence (CL) is reported. A drastic light emission enhancement is observed, related to a strong interaction between Cu2+ and the imidazolium ring. In these conditions, the CL reaction was able to produce light efficiently at pH as low as 6.5 (amplification factor: Intensity(+IL)/Intensity(-IL) = 2900). Interesting effects of [Emim][EtSO4]) on the enzyme glucose oxidase activity were also evidenced, and advantages were taken from this enhancement to perform sensitive chemiluminescent glucose detection (LOD = 4 mu M) at PH 8.0.

引用

页码：2401 / 2404

页数：4

共 33 条

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