IMMOBILIZED-METAL AFFINITY CHROMATOGRAPHY (IMAC): A REVIEW

被引：310

作者：

Block, Helena ^{[1
]}

Maertens, Barbara ^{[1
]}

Spriestersbach, Anne ^{[1
]}

Brinker, Nicole ^{[1
]}

Kubicek, Jan ^{[1
]}

Fabis, Roland ^{[1
]}

Labahn, Joerg ^{[2
]}

Schaefer, Frank ^{[1
]}

机构：

[1] QIAGEN GmbH, Hilden, Germany

[2] Res Ctr Julich, Inst Struct Biol IBI 2, Julich, Germany

来源：

GUIDE TO PROTEIN PURIFICATION, SECOND EDITION | 2009年 / 463卷

关键词：

HISTIDINE-TAGGED PROTEINS; ONE-STEP PURIFICATION; N-TERMINAL METHIONINE; RECOMBINANT PROTEINS; ESCHERICHIA-COLI; ENABLING TECHNOLOGIES; HIS-TAG; EXPRESSION; STRATEGIES; BINDING;

D O I：

10.1016/S0076-6879(09)63027-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of immunoglobulins and the Chelex method. The most relevant application-purification of histidine-tagged recombinant proteins-will be reviewed in greater detail with focus of state-of-the-art materials, methods, and protocols, and the limitations of IMAC and recent advances to improve the technology and the methods will be described.

引用

收藏

页码：439 / 473

页数：35

相关论文

共 102 条

[1] Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium [J].

Acton, TB ;

Gunsalus, KC ;

Xiao, R ;

Ma, LC ;

Aramini, J ;

Baran, MC ;

Chiang, YW ;

Climent, T ;

Cooper, B ;

Denissova, NG ;

Douglas, SM ;

Everett, JK ;

Ho, CK ;

Macapagal, D ;

Rajan, PK ;

Shastry, R ;

Shih, LY ;

Swapna, GVT ;

Wilson, M ;

Wu, M ;

Gerstein, M ;

Inouye, M ;

Hunt, JF ;

Montelione, GT .

NUCLEAR MAGNETIC RESONANCE OF BIOLOGICAL MACROMOLECULES, PART C, 2005, 394 :210-243

[2] ISOLATION OF PHOSPHOPROTEINS BY IMMOBILIZED METAL (FE-3+) AFFINITY-CHROMATOGRAPHY [J].

ANDERSSON, L ;

PORATH, J .

ANALYTICAL BIOCHEMISTRY, 1986, 154 (01) :250-254

[3] Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins [J].

Arnau, Jos ;

Lauritzen, Conni ;

Petersen, Gitte E. ;

Pedersen, John .

PROTEIN EXPRESSION AND PURIFICATION, 2006, 48 (01) :1-13

[4]

*BIOC INC, 2006, BIOC SURV REP

[5] Production and comprehensive quality control of recombinant human Interleukin-1β:: A case study for a process development strategy [J].

Block, Helena ;

Kubicek, Jan ;

Labahn, Joerg ;

Roth, Udo ;

Schaefer, Frank .

PROTEIN EXPRESSION AND PURIFICATION, 2008, 57 (02) :244-254

[6] RAPID ONE-STEP PURIFICATION OF GOAT IMMUNOGLOBULINS BY IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY [J].

BODEN, V ;

WINZERLING, JJ ;

VIJAYALAKSHMI, M ;

PORATH, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 181 (02) :225-232

[7] Structural analysis and classification of native proteins from E-coli commonly co-purified by immobilised metal affinity chromatography [J].

Bolanos-Garcia, Victor Martin ;

Davies, Owen Richard .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2006, 1760 (09) :1304-1313

[8]

Bornhorst JA, 2000, METHOD ENZYMOL, V326, P245

[9] Proteome-scale purification of human proteins from bacteria [J].

Braun, P ;

Hu, YH ;

Shen, BH ;

Halleck, A ;

Koundinya, M ;

Harlow, E ;

LaBaer, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (05) :2654-2659

[10] Expression of soluble recombinant proteins in a cell-free system using a 96-well format [J].

Busso, D ;

Kim, R ;

Kim, SH .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2003, 55 (03) :233-240

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