Differential Activity of Wnt/β-Catenin Signaling in the Embryonic Mouse Thalamus

被引：34
作者：
Bluske, Krista K. [2 ]
Kawakami, Yasuhiko [2 ,3 ]
Koyano-Nakagawa, Naoko [2 ]
Nakagawa, Yasushi [1 ,2 ]
机构：
[1] Univ Minnesota, Stem Cell Inst, Dept Neurosci, Grad Program Neurosci, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Ctr Dev Biol, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
来源：
DEVELOPMENTAL DYNAMICS | 2009年 / 238卷 / 12期
关键词：
thalamus; development; Wnt; Shh; Axin2; diencephalon; patterning; neurogenesis; olig3; CENTRAL-NERVOUS-SYSTEM; SONIC-HEDGEHOG; EXPRESSION PATTERNS; REGIONAL IDENTITY; GENE-EXPRESSION; NEURAL PLATE; FOREBRAIN; DISTINCT; PATHWAY; TRANSCRIPTION;
D O I：
10.1002/dvdy.22167
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
In neural development, several Wnt genes are expressed in the vertebrate diencephalon, including the thalamus. However, roles of Wnt signaling in the thalamus during neurogenesis are not well understood. We examined Wnt/beta-catenin activity in embryonic mouse thalamus and found that a Wnt target gene Axin2 and reporter activity of BAT-gal transgenic mice show similar, differential patterns within the thalamic ventricular zone, where ventral and rostral regions had lower activity than other regions. Expression of Wnt ligands and signaling components also showed complex, differential patterns. Finally, based on partially reciprocal patterns of Writ and Shh signals in the thalamic ventricular zone, we tested if Shh signal is sufficient or necessary for the differential Axin2 expression. Analysis of mice with enhanced or reduced Shh signal showed that Axin2 expression is similar to controls. These results suggest that differential Writ signaling may play a role in patterning the thalamus independent of Shh signaling. Developmental Dynamics 238:3297-3309, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码：3297 / 3309
页数：13
相关论文
共 43 条
[1]   Wnt canonical pathway restricts graded Shh/Gli patterning activity through the regulation of Gli3 expression [J].
Alvarez-Medina, Roberto ;
Cayuso, Jordi ;
Okubo, Tadashi ;
Takada, Shinji ;
Marti, Elisa .
DEVELOPMENT, 2008, 135 (02) :237-247
[2]   Hedgehog activation is required upstream of Wnt signalling to control neural progenitor proliferation [J].
Alvarez-Medina, Roberto ;
Le Dreau, Gwenvael ;
Ros, Marian ;
Marti, Elisa .
DEVELOPMENT, 2009, 136 (19) :3301-3309
[3]   Persistent and high levels of Hes1 expression regulate boundary formation in the developing central nervous system [J].
Baek, Joung Hee ;
Hatakeyama, Jun ;
Sakamoto, Susumu ;
Ohtsuka, Toshiyuki ;
Kageyama, Ryoichiro .
DEVELOPMENT, 2006, 133 (13) :2467-2476
[4]   Transgenic Wnt/TCF pathway reporters: all you need is Lef? [J].
Barolo, S. .
ONCOGENE, 2006, 25 (57) :7505-7511
[5]   Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling [J].
Bonner, Jennifer ;
Gribble, Suzanna L. ;
Veien, Eric S. ;
Nikolaus, O. Brant ;
Weidinger, Gilbert ;
Dorsky, Richard I. .
DEVELOPMENTAL BIOLOGY, 2008, 313 (01) :398-407
[6]   Wnt signaling is required at distinct stages of development for the induction of the posterior forebrain [J].
Braun, MM ;
Etheridge, A ;
Bernard, A ;
Robertson, CP ;
Roelink, H .
DEVELOPMENT, 2003, 130 (23) :5579-5587
[7]   THE MOUSE DLX-2 (TES-1) GENE IS EXPRESSED IN SPATIALLY RESTRICTED DOMAINS OF THE FOREBRAIN, FACE AND LIMBS IN MIDGESTATION MOUSE EMBRYOS [J].
BULFONE, A ;
KIM, HJ ;
PUELLES, L ;
PORTEUS, MH ;
GRIPPO, JF ;
RUBENSTEIN, JLR .
MECHANISMS OF DEVELOPMENT, 1993, 40 (03) :129-140
[8]  
Chenn Anjen, 2008, Organogenesis, V4, P76
[9]   WNTs in the vertebrate nervous system: From patterning to neuronal connectivity [J].
Ciani, L ;
Salinas, PC .
NATURE REVIEWS NEUROSCIENCE, 2005, 6 (05) :351-U17
[10]   Characterisation of the Wnt antagonists and their response to conditionally activated Wnt signalling in the developing mouse forebrain [J].
Diep, DB ;
Hoen, N ;
Backman, M ;
Machon, O ;
Krauss, S .
DEVELOPMENTAL BRAIN RESEARCH, 2004, 153 (02) :261-270
12345