Effect of magnesium ions on the thermal stability of human poly(A)-specific ribonuclease

被引：28
|
作者：
Liu, Wei-Feng
Zhang, Ao
Cheng, Yuan
Zhou, Hai-Meng [1 ]
Yan, Yong-Bin
机构：
[1] Tsinghua Univ, Dept Biol Sci & Biotechnol, Prot Sci Lab, Minist Educ, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
来源：
FEBS LETTERS | 2007年 / 581卷 / 05期
基金：
中国国家自然科学基金;
关键词：
poly(A) specific ribonuclease; thermal stability; divalent metal cation; thermal aggregation; mRNA turnover;
D O I：
10.1016/j.febslet.2007.02.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Poly(A)-specific ribonuclease (PARN), a member of the DEDD family, is a key enzyme involved in the deadenylation of mRNA in higher eukaryotic cells. In this research, it was found that Mg2+ could protect PARN against thermal inactivation by increasing the midpoint of inactivation and decreasing the inactivation rate. This protective effect was unique to Mg2+ in a concentration-dependent manner. However, the thermal unfolding and aggregation was promoted by the addition of Mg2+ at high temperatures. These results revealed that Mg2+ might have dual effects on PARN stability: protecting the active site but endangering the overall structural stability. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：1047 / 1052
页数：6
相关论文
共 50 条
  • [1] Allosteric regulation of human poly(A)-specific ribonuclease by cap and potassium ions
    Liu, Wei-Feng
    Zhang, Ao
    Cheng, Yuan
    Zhou, Hai-Meng
    Yan, Yong-Bin
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 379 (02) : 341 - 345
  • [2] Dissimilar Roles of the Four Conserved Acidic Residues in the Thermal Stability of Poly(A)-Specific Ribonuclease
    He, Guang-Jun
    Liu, Wei-Feng
    Yan, Yong-Bin
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 12 (05): : 2901 - 2916
  • [3] Conformational stability and multistate unfolding of poly(A)-specific ribonuclease
    He, Guang-Jun
    Zhang, Ao
    Liu, Wei-Feng
    Cheng, Yuan
    Yan, Yong-Bin
    FEBS JOURNAL, 2009, 276 (10) : 2849 - 2860
  • [4] Coordination of divalent metal ions in the active site of poly(A)-specific ribonuclease
    Ren, YG
    Kirsebom, LA
    Virtanen, A
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (47) : 48702 - 48706
  • [5] SOME SPECIFIC ION EFFECTS ON THE CONFORMATION AND THERMAL STABILITY OF RIBONUCLEASE
    GINSBURG, A
    CARROLL, WR
    FEDERATION PROCEEDINGS, 1965, 24 (2P1) : 412 - &
  • [6] Role of the RRM domain in the activity, structure and stability of poly(A)-specific ribonuclease
    Zhang, Ao
    Liu, Wei-Feng
    Yan, Yong-Bin
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 461 (02) : 255 - 262
  • [7] Effect of dopant ions on thermal stability of conducting poly(o-phenitidine)
    Patil, RC
    Radhakrishnan, S
    Ogura, K
    POLYMER DEGRADATION AND STABILITY, 2001, 73 (01) : 7 - 13
  • [8] Purification of poly(A)-specific ribonuclease
    Virtanen, A
    Martînez, J
    Ren, YG
    RIBONUCLEASES, PT B, 2001, 342 : 303 - 309
  • [9] EFFECT OF COFACTORS, OESTROGENS + MAGNESIUM IONS ON ACTIVITY + STABILITY OF HUMAN GLUTAMATE DEHYDROGENASE
    WARREN, JC
    CARR, DO
    GRISOLIA, S
    BIOCHEMICAL JOURNAL, 1964, 93 (02) : 409 - &
  • [10] Effect of natural nucleotides and their synthetic analogues on mRNA turnover by human poly(A)specific ribonuclease (PARN)
    Balatsos, N.
    Anastasakis, D.
    Maragozidis, P.
    Manta, S.
    Komiotis, D.
    Stathopoulos, C.
    FEBS JOURNAL, 2008, 275 : 117 - 117
12345