Glutathione-Responsive Multilayer Coated Gold Nanoparticles for Targeted Gene Delivery

被引：28
作者：
Yu, Feifei [1 ]
Huang, Jingbin [1 ]
Yu, Yuan [1 ]
Lu, Ying [1 ]
Chen, Yan [1 ]
Zhang, He [1 ]
Zhou, Guichen [3 ]
Sun, Zhiguo [1 ]
Liu, Junjie [1 ]
Sung, Duxin [2 ]
Zhang, Guoqing [3 ]
Zou, Hao [1 ]
Zhong, Yanqiang [1 ]
机构：
[1] Second Mil Med Univ, Sch Pharm, Dept Pharmaceut Sci, Shanghai 200433, Peoples R China
[2] Univ Michigan, Sch Pharm, Dept Pharmaceut Sci, Ann Arbor, MI 48109 USA
[3] Eastern Hepatobiliaty Surg Hosp, Dept Pharm, Shanghai 200438, Peoples R China
来源：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2016年 / 12卷 / 03期
关键词：
Gold Nanoparticles; pTAT; Hyaluronic Acid; Glutathione-Responsive; TAT-PEPTIDE; DRUG-DELIVERY; DNA; VECTORS; BIODISTRIBUTION; POLYETHYLENIMINE; AGENTS; LIVER; SIRNA;
D O I：
10.1166/jbn.2016.2177
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Efficient gene release after intracellular uptake is very important for non-viral gene delivery systems. To construct a glutathione-responsive gene delivery system, we developed gold-cysteamine (AuCM)/plasmid DNA (pDNA)/poly TAT (pTAT)/hyaluronic acid (HA) nanocomplexes (AuCM/pDNA/pTAT/HA) in this study. The AuCM/pDNA/pTAT/HA nanocomplexes possessed a small size less than 200 nm and negative zeta potential of -17 +/- 4 mV. The multilayer structure was verified by UV-Vis spectra, surface charges, dynamic light scattering. Morphology was observed by transmission electron microscope. The AuCM/pDNA/pTAT/HA nanocomplexes could completely protect pDNA against enzymatic degradation. These nanocomplexes showed effective cellular uptake in CD44 receptors over-expressed HepG 2 cells in a HA/CD44 interaction dependent manner. Moreover, transfection efficacy was significantly enhanced in AuCM/pDNA/pTAT/HA treated HepG 2 cells compared with AuCM/pDNA/pTAT, and was further enhanced in the presence of GSH, indicating that AuCM/pDNA/pTAT/HA was glutathione-responsive. Biodistribution revealed that AuCM/pDNA/pTAT/HA nanocomplexes mainly accumulated in liver. In conclusion, AuCM/pDNA/pTAT/HA nanocomplexes may serve as glutathione-responsive gene carriers for actively targeting gene delivery to CD44 receptors over-expressed liver cancers.
引用
收藏
页码：503 / 515
页数：13
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