The influence of heart valve leaflet matrix characteristics on the interaction between human mesenchymal stem cells and decellularized scaffolds

被引：72

作者：

Iop, Laura ^{[1
]}

Renier, Vera ^{[1
]}

Naso, Filippo ^{[2
]}

Piccoli, Martina ^{[3
]}

Bonetti, Antonella ^{[4
]}

Gandaglia, Alessandro ^{[1
]}

Pozzobon, Michela ^{[3
]}

Paolin, Adolfo ^{[5
]}

Ortolani, Fulvia ^{[4
]}

Marchini, Maurizio ^{[4
]}

Spina, Michele ^{[2
]}

De Coppi, Paolo ^{[3
,6
,7
]}

Sartore, Saverio ^{[8
]}

Gerosa, Gino ^{[1
]}

机构：

[1] Univ Padua, Sch Med, Dept Cardiol Thorac & Vasc Sci, I-35128 Padua, Italy

[2] Univ Padua, Sch Med, Dept Expt Biomed Sci, I-35100 Padua, Italy

[3] Univ Padua, Sch Med, Dept Pediat, I-35128 Padua, Italy

[4] Univ Udine, Sch Med, Dept Med & Morphol Res, I-33100 Udine, Italy

[5] Treviso Hosp, Tissue Bank, I-31100 Treviso, Italy

[6] UCL, Great Ormond St Hosp, Surg Unit, London WC1N 1EH, England

[7] UCL, Inst Child Hlth, London WC1N 1EH, England

[8] Univ Padua, Stem Cell Unit, I-35128 Padua, Italy

来源：

BIOMATERIALS | 2009年 / 30卷 / 25期

关键词：

Heart valve; Heart valve tissue engineering; Mesenchymal stem cells; Cell-ECM interactions; PORCINE AORTIC-VALVE; EXTRACELLULAR-MATRIX; INTERSTITIAL-CELLS; IMMUNOGENICITY; CALCIFICATION; PROLIFERATION; BIOPROSTHESES; MOLECULES; RELEVANCE; PATHWAY;

D O I：

10.1016/j.biomaterials.2009.04.031

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The potential for in vitro colonization of decellularized valves by human bone marrow mesenchymal stem cells (hBM-MSCs) towards the anisotropic layers ventricularis and fibrosa and in homo- vs. heterotypic cell-ECM interactions has never been investigated. hBM-MSCs were expanded and characterized by immunofluorescence and FACS analysis. Porcine and human pulmonary valve leaflets (p- and hPVLs, respectively) underwent decellularization with Triton X100-sodium cholate treatment (TRICOL), followed by nuclear fragment removal. hBM-MSCs (2 x 10(6) cells/cm(2)) were seeded onto fibrosa (FS) or ventricularis (VS) of decellularized PVLs, precoated with FBS and fibronectin, and statically cultured for 30 days. Bioengineered PVLs revealed no histopathological features but a reconstructed endothelium lining and the presence of fibroblasts, myofibroblasts and SMCs, as in the corresponding native leaflet. The two valve layers behaved differently as regards hBM-MSC repopulation potential, however, with a higher degree of 3D spreading and differentiation in VS than in FS samples, and with enhanced cell survival and colonization effects in the homotypic ventricularis matrix, suggesting that hBM-MSC phenotypic conversion is strongly influenced in vitro by the anisotropic valve microstructure and species-specific matching between extracellular matrix and donor cells. These findings are of particular relevance to in vivo future applications of valve tissue engineering. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：4104 / 4116

页数：13

共 41 条

[1] The immunogenicity of the extracellular matrix in arterial xenografts [J].

Allaire, E ;

Bruneval, P ;

Mandet, C ;

Becquemin, JP ;

Michel, JB .

SURGERY, 1997, 122 (01) :73-81

[2] IgG deposition and activation of the classical complement pathway involvement in the activation of human granulocytes by decellularized porcine heart valve tissue [J].

Bastian, Fabienne ;

Stelzmueller, Marie-Elisabeth ;

Kratochwill, Klaus ;

Kasimir, Marie-Theres ;

Simon, Paul ;

Weigel, Guenter .

BIOMATERIALS, 2008, 29 (12) :1824-1832

[3] Human mesenchymal stem cells induce T cell anergy and downregulate T cell allo-responses via the TH2 pathway: Relevance to tissue engineering human heart valves [J].

Batten, Puspa ;

Sarathchandra, Padmini ;

Antoniw, Joseph W. ;

Tay, Szun Szun ;

Lowdell, Mark W. ;

Taylor, Patricia M. ;

Yacoub, Magdi H. .

TISSUE ENGINEERING, 2006, 12 (08) :2263-2273

[4] Cell adhesion to fibrillin-1 molecules and microfibrils is mediated by α5β1 and αvβ3 integrins [J].

Bax, DV ;

Bernard, SE ;

Lomas, A ;

Morgan, A ;

Humphries, J ;

Shuttleworth, CA ;

Humphries, MJ ;

Kielty, CM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (36) :34605-34616

[5] Cell characterization of porcine aortic valve and decellularized leaflets repopulated with aortic valve interstitial cells: The VESALIO project (Vitalitate Exornatum Succedaneum Aorticum Labore Ingenioso Obtenibitur) [J].

Bertipaglia, B ;

Ortolani, F ;

Petrelli, L ;

Gerosa, G ;

Spina, M ;

Pauletto, P ;

Casarotto, D ;

Marchini, M ;

Sartore, S .

ANNALS OF THORACIC SURGERY, 2003, 75 (04) :1274-1282

[6] Construction of tissue-engineered homograft bioprosthetic heart valves in vitro [J].

Bin, Feng ;

Yinglong, Liu ;

Nin, Xie ;

Kai, Feng ;

Son, Laifeng ;

Zhu, Xiaodong .

ASAIO JOURNAL, 2006, 52 (03) :303-309

[7]

Birk D.E., 1994, Extracellular Matrix Assembly and Structure, P91, DOI DOI 10.1016/B978-0-12-775170-2.50009-3

[8] EXTRACELLULAR COMPARTMENTS IN TENDON MORPHOGENESIS - COLLAGEN FIBRIL, BUNDLE, AND MACROAGGREGATE FORMATION [J].

BIRK, DE ;

TRELSTAD, RL .

JOURNAL OF CELL BIOLOGY, 1986, 103 (01) :231-240

[9] Different patterns of extracellular matrix protein expression in the convexity and the concavity of the dilated aorta with bicuspid aortic valve: Preliminary results [J].

Cotrufo, M ;

Della Corte, A ;

De Santo, LS ;

Quarto, C ;

De Feo, M ;

Romano, G ;

Amarelli, C ;

Scardone, M ;

Di Meglio, F ;

Guerra, G ;

Scarano, M ;

Vitale, S ;

Castaldo, C ;

Montagnani, S .

JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 2005, 130 (02) :504-511

[10] Cell composition of the human pulmonary valve: A comparative study with the aortic valve - The VESALIO* project [J].

Della Rocca, F ;

Sartore, S ;

Guidolin, D ;

Bertiplaglia, B ;

Gerosa, G ;

Casarotto, D ;

Pauletto, P .

ANNALS OF THORACIC SURGERY, 2000, 70 (05) :1594-1600

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