Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury

被引：37
作者：
Xia, Chengcheng [1 ,2 ]
Chang, Pengyu [1 ,2 ,3 ]
Zhang, Yuyu [1 ,2 ]
Shi, Weiyan [1 ,2 ]
Liu, Ben [4 ]
Ding, Lijuan [1 ,2 ]
Liu, Min [1 ,2 ]
Gao, Ling [1 ,2 ]
Dong, Lihua [1 ,2 ]
机构：
[1] Jilin Univ, Bethune Hosp 1, Dept Radiat Oncol, Changchun 130000, Jilin, Peoples R China
[2] Jilin Univ, Sch Publ Hlth, Chinese Minist Hlth, Key Lab Radiobiol, Changchun 130000, Jilin, Peoples R China
[3] Acad Sci, Changchun Inst Appl Chem, Electrochem State Key Lab, Changchun 130000, Jilin, Peoples R China
[4] Jilin Univ, Bethune Hosp 1, Dept Orthoped, Changchun 130000, Jilin, Peoples R China
来源：
ONCOLOGY REPORTS | 2016年 / 35卷 / 02期
基金：
中国国家自然科学基金;
关键词：
radiation pneumonitis; radiation pulmonary fibrosis; thoracic tumor; radiotherapy; bone marrow-derived mesenchymal stem cells; STROMAL CELLS; RAT MODEL; TISSUE; MICE; PNEUMONITIS; IRRADIATION; ENGRAFTMENT; EXPRESSION; CYTOKINES; SEVERITY;
D O I：
10.3892/or.2015.4433
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Radiation-induced lung injury (RILI) is a fatal condition featured by interstitial pneumonitis and fibrosis. Mesenchymal stem cells (MSCs) have been widely used for treating RILI in rodent models. In the present study, we aimed to investigate whether the therapeutic effects of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) on RILI were in a dose-dependent manner. A total of 100 mice were randomly divided into: a control group (n=25), subject to lung irradiation and injection of phosphate-buffered solution (PBS) via the tail vein; and the hBM-MSC group, subject to lung irradiation followed by injection of a low dose (1x10(3) hBM-MSCs/g), medium dose (5x10(3) hBM-MSCs/g) and high dose (1x10(4) hBM-MSCs/g) of hBM-MSCs in PBS through the tail vein, respectively. After sacrifice, the pulmonary tissues were subject to hematoxylin and eosin (H&E) staining, Masson's trichrome staining and immunohistochemical staining to investigate the pathological changes. Immunofluorescent staining was performed to evaluate the differentiation capacity of hBM-MSCs in vivo by analyzing the expression of SPC and PECAM. hBM-MSCs improved the survival rate and histopathological features in the irradiated mice, especially in the low-dose group. Marked decrease in collagen deposition was noted in the irradiated mice treated using a low dose of hBM-MSCs. In addition, hBM-MSCs attenuated secretion and expression of IL-10 and increased the expression of TNF-alpha. Furthermore, hBM-MSCs had the potential to differentiate into functional cells upon lung injury. Low-dose hBM-MSCs contributed to functional recovery in mice with RILI.
引用
收藏
页码：731 / 738
页数：8
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