Single-molecule fluorescence studies of RNA: A decade's progress

被引：12

作者：

Karunatilaka, Krishanthi S. ^{[1
]}

Rueda, David ^{[1
]}

机构：

[1] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA

来源：

CHEMICAL PHYSICS LETTERS | 2009年 / 476卷 / 1-3期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

RESONANCE ENERGY-TRANSFER; TERTIARY STRUCTURE FORMATION; II INTRON RIBOZYME; HAIRPIN RIBOZYME; FOLDING LANDSCAPE; TRANSITION-STATE; STRUCTURED RNA; GAAA TETRALOOP; RIBONUCLEASE-P; L1; STALK;

D O I：

10.1016/j.cplett.2009.06.001

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Over the past decade, single-molecule fluorescence studies have elucidated the structure-function relationship of RNA molecules. The real-time observation of individual RNAs by single-molecule fluorescence has unveiled the dynamic behavior of complex RNA systems in unprecedented detail, revealing the presence of transient intermediate states and their kinetic pathways. This review provides an overview of how single-molecule fluorescence has been used to explore the dynamics of RNA folding and catalysis. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1 / 10

页数：10

共 96 条

[41] STRUCTURE AND ACTIVITIES OF GROUP-II INTRONS [J].

MICHEL, F ;

FERAT, JL .

ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :435-461

[42] Single-molecule fluorescence of nucleic acids [J].

Mollova, ET .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2002, 6 (06) :823-828

[43] Fluorescence from diffusing single molecules illuminates biomolecular structure and dynamics [J].

Mukhopadhyay, Samrat ;

Deniz, Ashok A. .

JOURNAL OF FLUORESCENCE, 2007, 17 (06) :775-783

[44] Identification of two distinct hybrid state intermediates on the ribosome [J].

Munro, James B. ;

Altman, Roger B. ;

O'Connor, Nathan ;

Blanchard, Scott C. .

MOLECULAR CELL, 2007, 25 (04) :505-517

[45] Observation of internal cleavage and ligation reactions of a ribozyme [J].

Nahas, MK ;

Wilson, TJ ;

Hohng, SC ;

Jarvie, K ;

Lilley, DMJ ;

Ha, T .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (11) :1107-1113

[46] Optical detection of single molecules [J].

Nie, SM ;

Zare, RN .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1997, 26 :567-596

[47] The structural basis of ribosome activity in peptide bond synthesis [J].

Nissen, P ;

Hansen, J ;

Ban, N ;

Moore, PB ;

Steitz, TA .

SCIENCE, 2000, 289 (5481) :920-930

[48] UNUSUAL RESISTANCE OF PEPTIDYL TRANSFERASE TO PROTEIN EXTRACTION PROCEDURES [J].

NOLLER, HF ;

HOFFARTH, V ;

ZIMNIAK, L .

SCIENCE, 1992, 256 (5062) :1416-1419

[49] RNA folding and unfolding [J].

Onoa, B ;

Tinoco, I .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2004, 14 (03) :374-379

[50] Identifying kinetic barriers to mechanical unfolding of the T-thermophila ribozyme [J].

Onoa, B ;

Dumont, S ;

Liphardt, J ;

Smith, SB ;

Tinoco, I ;

Bustamante, C .

SCIENCE, 2003, 299 (5614) :1892-1895

← 1 2 3 4 5 6 7 8 9 10 →