Diagnosis of snake envenomation using a simple phospholipase A2 assay

被引：34

作者：

Maduwage, Kalana ^{[1
,2
]}

O'Leary, Margaret A. ^{[3
]}

Isbister, Geoffrey K. ^{[1
,3
]}

机构：

[1] Univ Newcastle, Sch Med & Publ Hlth, Callaghan, NSW 2308, Australia

[2] Univ Peradeniya, Fac Med, Dept Biochem, Peradeniya, Sri Lanka

[3] Calvary Mater Newcastle, Dept Clin Toxicol & Pharmacol, Waratah, NSW 2298, Australia

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

基金：

英国医学研究理事会;

关键词：

COAGULOPATHY; SEPSIS; SERUM;

D O I：

10.1038/srep04827

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Diagnosis of snake envenomation is challenging but critical for deciding on antivenom use. Phospholipase A(2) enzymes occur commonly in snake venoms and we hypothesized that phospholipase activity detected in human blood post-bite may be indicative of envenomation. Using a simple assay, potentially a bedside test, we detected high phospholipase activity in sera of patients with viper and elapid envenomation compared to minimal activity in non-envenomed patients.

引用

页数：4

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