Gene expression profiling in anti-CD11d mAb-treated spinal cord-injured rats

被引：16
作者：
Gris, Paul [1 ,2 ]
Tighe, Allyson [1 ,3 ]
Thawer, Sakina [1 ,4 ]
Hemphill, Andrew [1 ]
Oatway, Mark [1 ,2 ]
Weaver, Lynne [1 ,2 ,3 ]
Dekaban, Gregory A. [1 ,2 ,4 ]
Brown, Arthur [1 ,2 ,5 ]
机构：
[1] Robarts Res Inst, BioTherapeut Res Grp, Spinal Cord Injury Team, London, ON N6A 5K8, Canada
[2] Univ Western Ontario, Neurosci Program, London, ON N6A 5K8, Canada
[3] Univ Western Ontario, Dept Physiol & Pharmacol, London, ON N6A 5K8, Canada
[4] Univ Western Ontario, Dept Microbiol & Immunol, London, ON N6A 5K8, Canada
[5] Univ Western Ontario, Dept Anat & Cell Biol, London, ON N6A 5K8, Canada
来源：
JOURNAL OF NEUROIMMUNOLOGY | 2009年 / 209卷 / 1-2期
基金：
加拿大健康研究院;
关键词：
Spinal cord injury; Genomics; Microarray; Gene expression profiling; Anti-inflammatory; CSPGs; MONOCLONAL-ANTIBODY; CHONDROITIN-SULFATE; MOLECULAR-CLONING; CELL-DEATH; GLIAL SCAR; MACROPHAGES; ACTIVATION; PROTEIN; INFLAMMATION; SEVERITY;
D O I：
10.1016/j.jneuroim.2009.02.002
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Acute administration of a mononclonal antibody (mAb) raised against the CD11d subunit of the leukocyte CD11d/CD18 integrin after spinal cord injury (SCI) in the rat greatly improves neurological outcomes. We have profiled gene expression in anti-CD11d and isotyped-matched control mAb-treated rats after SCI. Microarray analysis demonstrated reduced expression of pro-inflammatory cytokines and increased expression of inflammatory mediators that promote wound healing and the expression of scar proteins predicted to improve nerve growth. These changes in gene expression may reflect changes in the types of macrophages that populate the lesions in anti-CD11d mAb-treated rats. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：104 / 113
页数：10
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