A SCARECROW-RETINOBLASTOMA Protein Network Controls Protective Quiescence in the Arabidopsis Root Stem Cell Organizer

被引:130
作者
Cruz-Ramirez, Alfredo [1 ,2 ]
Diaz-Trivino, Sara [1 ]
Wachsman, Guy [1 ]
Du, Yujuan [1 ]
Arteaga-Vazquez, Mario [3 ]
Zhang, Hongtao [1 ]
Benjamins, Rene [1 ]
Blilou, Ikram [1 ]
Neef, Anne B. [4 ]
Chandler, Vicki [5 ,6 ]
Scheres, Ben [1 ]
机构
[1] Univ Utrecht, Dept Mol Genet, Utrecht, Netherlands
[2] Cinvestav Sede Irapuato, Lab Nacl Gen Biodiversidad, Irapuato, Mexico
[3] Univ Veracruzana, Inst Biotecnol & Ecol Aplicada INBIOTECA, Xalapa 91000, Veracruz, Mexico
[4] Univ Zurich, Inst Organ Chem, Zurich, Switzerland
[5] Univ Arizona, BIO5 Inst, Tucson, AZ USA
[6] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA
关键词
CYCLE PROGRESSION; CLONAL ANALYSIS; SELF-RENEWAL; DNA-DAMAGE; GENE; DIFFERENTIATION; DIVISION; MERISTEM; TRANSCRIPTION; MAINTENANCE;
D O I
10.1371/journal.pbio.1001724
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Quiescent long-term somatic stem cells reside in plant and animal stem cell niches. Within the Arabidopsis root stem cell population, the Quiescent Centre ( QC), which contains slowly dividing cells, maintains surrounding short-term stem cells and may act as a long-term reservoir for stem cells. The RETINOBLASTOMA-RELATED (RBR) protein cell-autonomously reinforces mitotic quiescence in the QC. RBR interacts with the stem cell transcription factor SCARECROW (SCR) through an LxCxE motif. Disruption of this interaction by point mutation in SCR or RBR promotes asymmetric divisions in the QC that renew short-term stem cells. Analysis of the in vivo role of quiescence in the root stem cell niche reveals that slow cycling within the QC is not needed for structural integrity of the niche but allows the growing root to cope with DNA damage.
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页数:12
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